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Macrophage Migration Inhibitory Factor pp 173–183Cite as

Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2080))

Abstract

Cell death is a vital process for maintaining tissue homeostasis and removing potentially harmful cells. Cell death can be both programmed and non-programmed and is commonly divided into two main forms, termed apoptotic and necrotic death modes. In this chapter cell death is classified into apoptosis, primary necrosis, pyroptosis, and necroptosis. This chapter outlines the measurement of these different types of cell death and the relationship of measuring MIF release in these assays.

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References

  1. Calandra T, Roger T (2003) Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol 3:791

    Article  CAS  Google Scholar 

  2. Bloom J, Sun S, Al-Abed Y (2016) MIF, a controversial cytokine: a review of structural features, challenges, and opportunities for drug development. Expert Opin Ther Targets 20(12):1463–1475

    Article  CAS  Google Scholar 

  3. Calandra T et al (1994) The macrophage is an important and previously unrecognized source of macrophage migration inhibitory factor. J Exp Med 179(6):1895–1902

    Article  CAS  Google Scholar 

  4. Rice EK et al (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II. Kidney Int 63(4):1265–1275

    Article  CAS  Google Scholar 

  5. Calandra T et al (2000) Protection from septic shock by neutralization of macrophage migration inhibitory factor. Nat Med 6:164

    Article  CAS  Google Scholar 

  6. Radstake TRDJ et al (2005) Correlation of rheumatoid arthritis severity with the genetic functional variants and circulating levels of macrophage migration inhibitory factor. Arthritis Rheum 52(10):3020–3029

    Article  CAS  Google Scholar 

  7. Kim HR et al (2007) Macrophage migration inhibitory factor upregulates angiogenic factors and correlates with clinical measures in rheumatoid arthritis. J Rheumatol 34(5):927–936

    CAS  PubMed  Google Scholar 

  8. Foote A et al (2004) Macrophage migration inhibitory factor in systemic lupus erythematosus. J Rheumatol 31(2):268–273

    CAS  PubMed  Google Scholar 

  9. Sánchez E et al (2006) Evidence of association of macrophage migration inhibitory factor gene polymorphisms with systemic lupus erythematosus. Genes Immun 7:433

    Article  Google Scholar 

  10. Kok T et al (2018) Small-molecule inhibitors of macrophage migration inhibitory factor (MIF) as an emerging class of therapeutics for immune disorders. Drug Discov Today 23(11):1910–1918

    Article  CAS  Google Scholar 

  11. Hertelendy J et al (2018) Macrophage migration inhibitory factor - a favorable marker in inflammatory diseases? Curr Med Chem 25(5):601–605

    Article  CAS  Google Scholar 

  12. Roth S et al (2015) Secondary necrotic neutrophils release interleukin-16C and macrophage migration inhibitory factor from stores in the cytosol. Cell Death Discov 1:15056–15056

    Article  CAS  Google Scholar 

  13. Roth S, Solbach W, Laskay T (2016) IL-16 and MIF: messengers beyond neutrophil cell death. Cell Death Dis 7(1):e2049–e2049

    Article  CAS  Google Scholar 

  14. Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35(4):495–516

    Article  CAS  Google Scholar 

  15. Jorgensen I, Rayamajhi M, Miao EA (2017) Programmed cell death as a defence against infection. Nat Rev Immunol 17:151

    Article  CAS  Google Scholar 

  16. Cohen JJ (1991) Programmed cell death in the immune system. Adv Immunol 50:55–85

    Article  CAS  Google Scholar 

  17. Yang Y et al (2015) Programmed cell death and its role in inflammation. Mil Med Res 2(1):12

    Article  Google Scholar 

  18. Choi JJ, Reich Iii CF, Pisetsky DS (2004) Release of DNA from dead and dying lymphocyte and monocyte cell lines in vitro. Scand J Immunol 60(1–2):159–166

    Article  CAS  Google Scholar 

  19. Vermes I et al (1995) A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184(1):39–51

    Article  CAS  Google Scholar 

  20. Yurinskaya V et al (2017) A comparative study of U937 cell size changes during apoptosis initiation by flow cytometry, light scattering, water assay and electronic sizing. Apoptosis 22(10):1287–1295

    Article  CAS  Google Scholar 

  21. Castilla R et al (2004) Dual effect of ethanol on cell death in primary culture of human and rat hepatocytes. Alcohol Alcohol 39(4):290–296

    Article  CAS  Google Scholar 

  22. Tait SWG, Ichim G, Green DR (2014) Die another way--non-apoptotic mechanisms of cell death. J Cell Sci 127(Pt 10):2135–2144

    Article  CAS  Google Scholar 

  23. Bergsbaken T, Fink SL, Cookson BT (2009) Pyroptosis: host cell death and inflammation. Nat Rev Microbiol 7(2):99–109

    Article  CAS  Google Scholar 

  24. Man SM, Karki R, Kanneganti TD (2017) Molecular mechanisms and functions of pyroptosis, inflammatory caspases and inflammasomes in infectious diseases. Immunol Rev 277(1):61–75

    Article  CAS  Google Scholar 

  25. Magna M, Pisetsky D (2015) The role of cell death in the pathogenesis of SLE: Is pyroptosis the missing link? Scand J Immunol 82(3):218–224

    Article  CAS  Google Scholar 

  26. Aglietti RA, Dueber EC (2017) Recent insights into the molecular mechanisms underlying pyroptosis and gasdermin family functions. Trends Immunol 38(4):261–271

    Article  CAS  Google Scholar 

  27. Vanaja SK, Rathinam VA, Fitzgerald KA (2015) Mechanisms of inflammasome activation: recent advances and novel insights. Trends Cell Biol 25(5):308–315

    Article  CAS  Google Scholar 

  28. He Y, Hara H, Nunez G (2016) Mechanism and regulation of NLRP3 inflammasome activation. Trends Biochem Sci 41(12):1012–1021

    Article  CAS  Google Scholar 

  29. Lang T et al (2018) Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation. Nat Commun 9(1):2223

    Article  Google Scholar 

  30. Dhuriya YK, Sharma D (2018) Necroptosis: a regulated inflammatory mode of cell death. J Neuroinflammation 15(1):199

    Article  Google Scholar 

  31. Omoto S et al (2015) Suppression of RIP3-dependent necroptosis by human cytomegalovirus. J Biol Chem 290(18):11635–11648

    Article  CAS  Google Scholar 

  32. Murphy JM, Silke J (2014) Ars Moriendi; the art of dying well–new insights into the molecular pathways of necroptotic cell death. EMBO Rep 15(2):155–164

    Article  CAS  Google Scholar 

  33. Hanson B (2016) Necroptosis: a new way of dying? Cancer Biol Ther 17(9):899–910

    Article  CAS  Google Scholar 

  34. Kearney CJ, Martin SJ (2017) An inflammatory perspective on necroptosis. Mol Cell 65(6):965–973

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Monash University, Clayton, VIC, Australia

    Shahrzad Zamani

  2. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing & Health Sciences, Monash University, Monash Medical Centre, Clayton, VIC, Australia

    Eric F. Morand & Jacqueline K. Flynn

Authors
  1. Shahrzad Zamani
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  2. Eric F. Morand
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  3. Jacqueline K. Flynn
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Corresponding author

Correspondence to Eric F. Morand .

Editor information

Editors and Affiliations

  1. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    James Harris

  2. Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, VIC, Australia

    Eric F. Morand

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Zamani, S., Morand, E.F., Flynn, J.K. (2020). Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release. In: Harris, J., Morand, E. (eds) Macrophage Migration Inhibitory Factor. Methods in Molecular Biology, vol 2080. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9936-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9936-1_15

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9935-4

  • Online ISBN: 978-1-4939-9936-1

  • eBook Packages: Springer Protocols

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