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Generation of High-Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2073))

Abstract

Molecularly imprinted polymers are leading technology in the development of protein biomimetics. This chapter describes the protocol for the synthesis of protein imprinted nanoparticles. These materials exhibit exceptional affinity (into the nM/pM range) and selectivity for their target template. The nanoparticles can be developed for a wide range of targets, while exhibiting excellent robustness, solubility, and flexibility in use. They are finding use in the creation of drug delivery vectors and sensing and recognition assays.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Leicester, Leicester, UK

    Francesco Canfarotta & Sergey A. Piletsky

  2. School of Pharmacy, De Montfort University, Leicester, UK

    Nicholas W. Turner

Authors
  1. Francesco Canfarotta
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  2. Sergey A. Piletsky
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  3. Nicholas W. Turner
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Corresponding author

Correspondence to Nicholas W. Turner .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

    Juliet A. Gerrard

  2. School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

    Laura J. Domigan

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Canfarotta, F., Piletsky, S.A., Turner, N.W. (2020). Generation of High-Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol. In: Gerrard, J., Domigan, L. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 2073. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9869-2_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9868-5

  • Online ISBN: 978-1-4939-9869-2

  • eBook Packages: Springer Protocols

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