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Immobilization of Enzymes and Microorganisms

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Basic Techniques in Biochemistry, Microbiology and Molecular Biology

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

Immobilization in polyacrylamide gel is a technique based on the polymerization of acrylamide with N,N′-methylene-bis-acrylamide (Bis) as the cross-linking agent. The degree of cross-linking is controlled by adjusting the ratio of acrylamide to Bis used. Being nonionic in nature, polyacrylamide is the most widely used matrix for entrapping enzymes.

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Jain, A., Jain, R., Jain, S. (2020). Immobilization of Enzymes and Microorganisms. In: Basic Techniques in Biochemistry, Microbiology and Molecular Biology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9861-6_39

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  • DOI: https://doi.org/10.1007/978-1-4939-9861-6_39

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9860-9

  • Online ISBN: 978-1-4939-9861-6

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