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Oncolytic Viruses pp 191–211Cite as

Detection of Tumor Antigen-Specific T-Cell Responses After Oncolytic Vaccination

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2058))

Abstract

Oncolytic vaccines, which consist of recombinant oncolytic viruses (OV) encoding tumor-associated antigens (TAAs), have demonstrated potent antitumor efficacy in preclinical models and are currently evaluated in phase I/II clinical trials. On one hand, oncolysis of OV-infected malignant entities reinstates cancer immunosurveillance. On the other hand, overexpression of TAAs in infected cells further stimulates the adaptive arm of antitumor immunity. Particularly, the presence of tumor-specific CD8+ T lymphocytes within the tumor microenvironment, as well as in the periphery, has demonstrated prognostic value for cancer treatments. These effector CD8+ T cells can be detected through their production of the prototypical Tc1 cytokine: IFN-γ. The quantitative and qualitative assessment of this immune cell subset remains critical in the development process of efficient cancer vaccines, including oncolytic vaccines. The present chapter will describe a single-cell immunological assay, namely the intracellular cytokine staining (ICS), that allows the enumeration of IFN-γ-producing TAA-specific CD8+ T cells in various tissues (tumor, blood, lymphoid organs) following oncolytic vaccination.

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Acknowledgments

J.G.P. was supported by the Seerave Foundation. B.W.B. was supported by the Terry Fox Research Institute. B.D.L. was supported by the Terry Fox Foundation, the Ontario Institute for Cancer Research, BioCanRx, and Turnstone Biologics.

Declaration of Interests: J.G.P., B.W.B., and B.D.L. share ownership of patents for cancer vaccination involving oncolytic vaccines. B.D.L. is cofounder and equity holder of and is on the board of directors of Turnstone Biologics.

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Authors and Affiliations

  1. Gustave Roussy Comprehensive Cancer Institute, Villejuif, France

    Jonathan G. Pol

  2. INSERM, U1138, Paris, France

    Jonathan G. Pol

  3. Equipe 11 Labellisée par la Ligue Nationale Contre le Cancer, Centre de Recherche des Cordeliers, Paris, France

    Jonathan G. Pol

  4. Université de Paris, Paris, France

    Jonathan G. Pol

  5. Sorbonne Université, Paris, France

    Jonathan G. Pol

  6. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada

    Byram W. Bridle

  7. Department of Pathology and Molecular Medicine, McMaster Immunology Research Centre, McMaster University, Hamilton, ON, Canada

    Brian D. Lichty

  8. Turnstone Biologics, Ottawa, ON, Canada

    Brian D. Lichty

Authors
  1. Jonathan G. Pol
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  2. Byram W. Bridle
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  3. Brian D. Lichty
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Corresponding author

Correspondence to Brian D. Lichty .

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Editors and Affiliations

  1. Research Group Mechanisms of Oncolytic Immunotherapy, Clinical Cooperation Unit Virotherapy, National Center for Tumor Diseases (NCT), German Cancer Research Center (DKFZ), University Hospital Heidelberg, Heidelberg, Germany

    Christine E. Engeland

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Pol, J.G., Bridle, B.W., Lichty, B.D. (2020). Detection of Tumor Antigen-Specific T-Cell Responses After Oncolytic Vaccination. In: Engeland, C. (eds) Oncolytic Viruses. Methods in Molecular Biology, vol 2058. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9794-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9794-7_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9793-0

  • Online ISBN: 978-1-4939-9794-7

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