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In Vitro Differentiation of T-Cells pp 93–106Cite as

In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2048))

Abstract

In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.

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References

  1. Morizane A, Doi D, Kikuchi T et al (2013) Direct comparison of autologous and allogeneic transplantation of iPSC-derived neural cells in the brain of a non-human primate. Stem Cell Reports 1:283–292. https://doi.org/10.1016/j.stemcr.2013.08.007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Chong JJH, Yang X, Don CW et al (2014) Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts. Nature 510:273–277. https://doi.org/10.1038/nature13233

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kawamura T, Miyagawa S, Fukushima S et al (2016) Cardiomyocytes Derived from MHC-homozygous induced pluripotent stem cells exhibit reduced allogeneic immunogenicity in MHC-matched non-human Primates. Stem Cell Reports 6:312–320. https://doi.org/10.1016/j.stemcr.2016.01.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Shiba Y, Gomibuchi T, Seto T et al (2016) Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538:388–391. https://doi.org/10.1038/nature19815

    Article  CAS  PubMed  Google Scholar 

  5. Sugita S, Iwasaki Y, Makabe K et al (2016) Successful transplantation of retinal pigment epithelial Cells from MHC homozygote iPSCs in MHC-matched models. Stem Cell Reports 7:635–648. https://doi.org/10.1016/j.stemcr.2016.08.010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Kikuchi T, Morizane A, Doi D et al (2017) Human iPS cell-derived dopaminergic neurons function in a primate Parkinson’s disease model. Nature 548:592–596. https://doi.org/10.1038/nature23664

    Article  CAS  PubMed  Google Scholar 

  7. Morizane A, Kikuchi T, Hayashi T et al (2017) MHC matching improves engraftment of iPSC-derived neurons in non-human primates. Nat Commun 8:385. https://doi.org/10.1038/s41467-017-00926-5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Hong SG, Winkler T, Wu C et al (2014) Path to the clinic: assessment of iPSC-BasedCell therapies in vivo in a nonhuman primate model. CellReports 7:1298–1309. https://doi.org/10.1016/j.celrep.2014.04.019

    Article  CAS  Google Scholar 

  9. Gori JL, Butler JM, Chan Y-Y et al (2015) Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells. J Clin Invest. https://doi.org/10.1172/JCI79328DS1

  10. D'Souza SS, Maufort J, Kumar A et al (2016) GSK3β inhibition promotes efficient myeloid and lymphoid Hematopoiesis from non-human primate-induced pluripotent stem cells. Stem Cell Reports 6:243–256. https://doi.org/10.1016/j.stemcr.2015.12.010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Kardel MD, Eaves CJ (2012) Modeling human hematopoietic cell development from pluripotent stem cells. Exp Hematol 40:601–611. https://doi.org/10.1016/j.exphem.2012.04.001

    Article  CAS  PubMed  Google Scholar 

  12. Kennedy M, D'Souza SL, Lynch-Kattman M et al (2007) Development of the hemangioblast defines the onset of hematopoiesis in human ES cell differentiation cultures. Blood 109:2679–2687. https://doi.org/10.1182/blood-2006-09

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Vodyanik MA, Thomson JA, Slukvin II (2006) Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood 108:2095–2105. https://doi.org/10.1182/blood-2006-02-003327

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Vizcardo R, Masuda K, Yamada D et al (2013) Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells. Cell Stem Cell 12:31–36. https://doi.org/10.1016/j.stem.2012.12.006

    Article  CAS  PubMed  Google Scholar 

  15. Nishimura T, Kaneko S, Kawana-Tachikawa A et al (2013) Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. Cell Stem Cell 12:114–126. https://doi.org/10.1016/j.stem.2012.11.002

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We thank Mr. Yasuyuki Miyake, Shuichi Kitayama, and Dr. Kahoru Taya for help in the preparation. This work is supported in part by KAKENHI to S.I. and S.K.

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Authors and Affiliations

  1. Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Research and Application, Kyoto University, Kyoto, Japan

    Shoichi Iriguchi

  2. Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Shin Kaneko

Authors
  1. Shoichi Iriguchi
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  2. Shin Kaneko
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Corresponding author

Correspondence to Shin Kaneko .

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Editors and Affiliations

  1. Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan

    Shin Kaneko

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Iriguchi, S., Kaneko, S. (2019). In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells. In: Kaneko, S. (eds) In Vitro Differentiation of T-Cells. Methods in Molecular Biology, vol 2048. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9728-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9728-2_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9727-5

  • Online ISBN: 978-1-4939-9728-2

  • eBook Packages: Springer Protocols

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