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Stable Isotope Probing of Microorganisms in Environmental Samples with H218O

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Book cover Stable Isotope Probing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2046))

Abstract

We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with H218O. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled with 18O. By measuring how much the DNA of each taxon becomes enriched with 18O when an environmental sample is incubated with H218O, it is feasible to quantify that population’s DNA replication rate, which is a proxy for growth.

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References

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Acknowledgments

This research was supported by the National Science Foundation (NSF DEB-1241094) and the Department of Energy’s Biological Systems Science Division, Program in Genomic Science (DE-SC0010579 and DE-SC0016207).

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Authors and Affiliations

  1. Department of Biological Sciences, Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, AZ, USA

    Egbert Schwartz, Michaela Hayer, Bruce A. Hungate & Rebecca L. Mau

  2. Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA

    Rebecca L. Mau

Authors
  1. Egbert Schwartz
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  2. Michaela Hayer
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  3. Bruce A. Hungate
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  4. Rebecca L. Mau
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Corresponding author

Correspondence to Egbert Schwartz .

Editor information

Editors and Affiliations

  1. School of Biological Sciences, University of Southampton, Southampton, UK

    Marc G. Dumont

  2. School of Biological Sciences, University of Southampton, Southampton, UK

    Marcela Hernández García

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Schwartz, E., Hayer, M., Hungate, B.A., Mau, R.L. (2019). Stable Isotope Probing of Microorganisms in Environmental Samples with H218O. In: Dumont, M., Hernández García, M. (eds) Stable Isotope Probing. Methods in Molecular Biology, vol 2046. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9721-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9721-3_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9720-6

  • Online ISBN: 978-1-4939-9721-3

  • eBook Packages: Springer Protocols

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