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ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader

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Purinergic Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2041))

Abstract

Imbalance in extracellular ATP levels in brain tissue has been suggested as a triggering factor for several neurological disorders. Here, we describe the most sensitive and reliable technique for monitoring the ATP levels in mice cerebrospinal samples collected by cisterna magna puncture technique and quantified using a microplate reader.

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Acknowledgments

This work was supported by funding from Spanish Ministry of Science and Education BFU2012-31195 to M.D.-H. European Union project H2020-MSCA-ITN-2017 number 766124 to M.D.-H. and from Universidad Complutense of Madrid (UCM)-Santander Central Hispano Bank PR41/17-21014 to M.D.-H. A.S.-S. was supported by BFU2012-31195 grant, and L.d.D.-G. held a UCM predoctoral fellowship supervised by M.D.-H. C.d.L. and C.B. were hired for an H2020-MSCA-ITN action supervised by M.D.-H.

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Author notes

  1. Laura de Diego-García and Álvaro Sebastián-Serrano contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Veterinary School, Complutense University of Madrid, Madrid, Spain

    Laura de Diego-García, Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro & Miguel Díaz-Hernández

  2. Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain

    Laura de Diego-García, Álvaro Sebastián-Serrano, Carolina Bianchi, Caterina Di Lauro & Miguel Díaz-Hernández

  3. Department of Physiology and Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland

    Laura de Diego-García

  4. FutureNeuro Research Center, Dublin, Ireland

    Laura de Diego-García

  5. Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Madrid, Spain

    Álvaro Sebastián-Serrano

Authors
  1. Laura de Diego-García
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  2. Álvaro Sebastián-Serrano
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  3. Carolina Bianchi
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  4. Caterina Di Lauro
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  5. Miguel Díaz-Hernández
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Corresponding author

Correspondence to Miguel Díaz-Hernández .

Editor information

Editors and Affiliations

  1. BioMedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain

    Pablo Pelegrín

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de Diego-García, L., Sebastián-Serrano, Á., Bianchi, C., Di Lauro, C., Díaz-Hernández, M. (2020). ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9717-6_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9716-9

  • Online ISBN: 978-1-4939-9717-6

  • eBook Packages: Springer Protocols

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