Abstract
The present protocol describes in detail the steps necessary for executing two highly versatile and minimally invasive surgical approaches for localized delivery of compounds to the central nervous system. The procedures have been designed for use on laboratory mice but can also be tailored for experimentations involving other small rodent models. Following the instructions outlined below, treatments can either be administered through single injections or infused over a longer period of time, at locations identified through stereotaxic coordinates, which ensure efficient targeting of the brain region of interest, as well as increased reproducibility between surgeries. Although the surgical interventions are well tolerated by laboratory animals, it is recommended to closely monitor the mice postoperatively for a few days, and take the necessary measures to prevent stress and discomfort.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Yamazaki Y, Hirai Y, Miyake K, Shimada T (2014) Targeted gene transfer into ependymal cells through intraventricular injection of AAV1 vector and long-term enzyme replacement via the CSF. Sci Rep 4:5506. https://doi.org/10.1038/srep05506
Barnabe-Heider F, Meletis K, Eriksson M, Bergmann O, Sabelstrom H, Harvey MA, Mikkers H, Frisen J (2008) Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation. Nat Methods 5(2):189–196. https://doi.org/10.1038/nmeth.1174
Sztainberg Y, Chen HM, Swann JW, Hao S, Tang B, Wu Z, Tang J, Wan YW, Liu Z, Rigo F, Zoghbi HY (2015) Reversal of phenotypes in MECP2 duplication mice using genetic rescue or antisense oligonucleotides. Nature 528(7580):123–126. https://doi.org/10.1038/nature16159
Schoch KM, DeVos SL, Miller RL, Chun SJ, Norrbom M, Wozniak DF, Dawson HN, Bennett CF, Rigo F, Miller TM (2016) Increased 4R-tau induces pathological changes in a human-tau mouse model. Neuron 90(5):941–947. https://doi.org/10.1016/j.neuron.2016.04.042
Grathwohl SA, Jucker M (2013) Replacement of osmotic minipumps to extend the intracerebral infusion time of compounds into the mouse brain. BioTechniques 55(2):75–78. https://doi.org/10.2144/000114064
Keith BJ, Franklin M, Paxinos G (2008) The mouse brain in stereotaxic coordinates, compact: the coronal plates and diagrams. Elsevier Science
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zamboni, M. (2019). Injection and Infusion of Compounds to the Central Nervous System. In: Gissberg, O., Zain, R., Lundin, K. (eds) Oligonucleotide-Based Therapies. Methods in Molecular Biology, vol 2036. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9670-4_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9670-4_14
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9669-8
Online ISBN: 978-1-4939-9670-4
eBook Packages: Springer Protocols