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Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells

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Progenitor Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2029))

Abstract

Flow cytometry is a widely used laser-based technology for rapid analysis of the expression of cell surface antigens and intracellular molecules in various cell types including hematopoietic stem/progenitor cells (HSPCs). Multiparametric analysis of individual cells within a short time frame makes this tool attractive and indispensable in the field of stem cell research. This is accomplished by harnessing the specific light scattering ability of the cell type, which determines its size and internal complexity. In addition, use of fluorescently conjugated antibodies allows the detection of a specific surface or intracellular antigen present at that particular stage. Fluorescent Activated Cell Sorting (FACS) is used to separate a subset of cells from a heterogeneous cell population based on fluorescent labeling. Here we describe the general principles of flow cytometry and detailed methods for the isolation of HSPCs using flow cytometry as a tool.

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Author information

Authors and Affiliations

  1. Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Sangeetha Vadakke-Madathil & Hina W. Chaudhry

  2. National Centre for Cell Sciences, Pune, India

    Lalita S. Limaye & Vaijayanti P. Kale

  3. Symbiosis Center for Stem Cell Research, Symbiosis School of Biological Sciences, Pune, India

    Vaijayanti P. Kale

Authors
  1. Sangeetha Vadakke-Madathil
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  2. Lalita S. Limaye
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  3. Vaijayanti P. Kale
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  4. Hina W. Chaudhry
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Corresponding author

Correspondence to Sangeetha Vadakke-Madathil .

Editor information

Editors and Affiliations

  1. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Mugdha V. Joglekar

  2. NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW, Australia

    Anandwardhan A. Hardikar

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Vadakke-Madathil, S., Limaye, L.S., Kale, V.P., Chaudhry, H.W. (2019). Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells. In: Joglekar, M., Hardikar, A. (eds) Progenitor Cells. Methods in Molecular Biology, vol 2029. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9631-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9631-5_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9630-8

  • Online ISBN: 978-1-4939-9631-5

  • eBook Packages: Springer Protocols

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