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Psychiatric Disorders pp 429–439Cite as

Methods in Electrode Implantation and Wiring for Long-Term Continuous EEG Monitoring in Rodent Models of Epilepsy and Behavioral Disturbances

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2011))

Abstract

Rodent seizure models that pathologically and behaviorally recapitulate age-tailored epileptic disorders are used by us and others to advance our understanding of the chronobiology and mechanisms of epileptic seizure emergence and their comorbidities and to investigate potential novel treatment strategies. Obtaining prolonged continuous electroencephalogram (EEG) tracings over months is essential in this line of translational research, particularly to assess the relation between electrographic changes and the development of seizures and their various psychiatric and cognitive comorbidities in models where seizures gradually emerge over weeks following brain insults. Here we describe our approach to electrode implantation and wiring in order to successfully obtain high-quality continuous EEG tracings in rats for prolonged periods. A detailed stepwise methodological description is provided with a special focus on the details that help most in avoiding notorious pitfalls such as premature EEG cable disconnections and a poor signal to noise ratio.

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Acknowledgments

Yasser Fadlallah was involved in this research under the Medical Research Volunteer Program (MRVP) at the American University of Beirut.

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Authors and Affiliations

  1. Department of Anatomy, Cell Biology and Physiology, American University of Beirut, Beirut, Lebanon

    Yasser Medlej, Houssein Salah, Rita Asdikian & Makram Obeid

  2. Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Lara Wadi & Zahraa Atoui

  3. Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon

    Yasser Fadlallah

  4. Animal Care Facility, American University of Beirut, Beirut, Lebanon

    Rana Bou Khalil & Rabih Hashash

  5. Division of Child Neurology, Department of Pediatric and Adolescent Medicine, American University of Beirut Medical Center, Beirut, Lebanon

    Makram Obeid

Authors
  1. Yasser Medlej
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  2. Houssein Salah
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  3. Lara Wadi
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  4. Zahraa Atoui
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  5. Yasser Fadlallah
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  6. Rita Asdikian
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  7. Rana Bou Khalil
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  8. Rabih Hashash
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  9. Makram Obeid
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Corresponding author

Correspondence to Makram Obeid .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon

    Firas H. Kobeissy

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Medlej, Y. et al. (2019). Methods in Electrode Implantation and Wiring for Long-Term Continuous EEG Monitoring in Rodent Models of Epilepsy and Behavioral Disturbances. In: Kobeissy, F. (eds) Psychiatric Disorders. Methods in Molecular Biology, vol 2011. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9554-7_25

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  • DOI: https://doi.org/10.1007/978-1-4939-9554-7_25

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9553-0

  • Online ISBN: 978-1-4939-9554-7

  • eBook Packages: Springer Protocols

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