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Streptococcus pneumoniae pp 101–111Cite as

Protein Expression Analysis by Western Blot and Protein–Protein Interactions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1968))

Abstract

Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections.

Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic markers. Here, we describe the detection of two pneumococcal proteins, pneumolysin and PpmA, in human urine by using monoclonal and polyclonal antibodies.

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Acknowledgments

The authors acknowledge Dr. Federico Iovino from Karolinska Institute for inviting us to write this chapter and for reviewing the manuscript, and Dr. Fermín Torrano for his constant support in the preparation of this chapter.

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Authors and Affiliations

  1. Escuela Superior de Ingeniería y Tecnología (ESIT), Universidad Internacional de La Rioja (UNIR), Logroño, Spain

    María Dolores Cima-Cabal & María del Mar García-Suárez

  2. Departamento de Microbiología, Hospital Universitario Central de Asturias & Fundación para la Investigación y la Innovación Biosanitaria del Principado de Asturias (FINBA), Oviedo, Spain

    Fernando Vazquez

  3. Departamento de Biología Funcional & Ophthalmology, Vision Sciences and Advanced Therapies Research Group, Instituto Universitario Fernández-Vega, Universidad de Oviedo, Oviedo, Spain

    Fernando Vazquez

  4. Área de Inmunología, Facultad de Medicina y Ciencias de la Salud, Universidad de Oviedo, Oviedo, Spain

    Juan R. de los Toyos

Authors
  1. María Dolores Cima-Cabal
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  2. Fernando Vazquez
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  3. Juan R. de los Toyos
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  4. María del Mar García-Suárez
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Corresponding author

Correspondence to María del Mar García-Suárez .

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Editors and Affiliations

  1. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Bioclinicum, Stockholm, Sweden

    Federico Iovino

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Cima-Cabal, M.D., Vazquez, F., de los Toyos, J.R., del Mar García-Suárez, M. (2019). Protein Expression Analysis by Western Blot and Protein–Protein Interactions. In: Iovino, F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9199-0_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9198-3

  • Online ISBN: 978-1-4939-9199-0

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