Abstract
Animal models are fundamental tools to study the biology of physiological processes and disease pathogenesis. To study invasive pneumococcal disease (IPD), many models using mice in particular have been established and developed during recent years. Thanks to the advances of the research in the pneumococcal field, nowadays, there is the possibility to use defined mouse models to study each disease caused by the pneumococcus. In this chapter mouse models for pneumonia, bacteremia, and meningitis are described. Since pneumococci are commensal pathogens found to a high extent in healthy individuals. Hence, we also describe a mouse model for nasopharyngeal colonization.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Troeger C, Blacker BF, Khalil IA, Rao PC, Cao S, Zimsen SRM, Albertson S, Stanaway JD, Deshpande A, Farag T, et al (2018) Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Infect Dis. 18(11):1191–1210. Published online 2018/09/24 https://doi.org/10.1016/S1473-3099(18)30310-4
Malley R, Weiser JN (2008) Animal models of pneumococcal colonization. In: Siber GR, Klugman KP, Mäkelä PH (ed.) Pneumococcal vaccines: the impact of coniugate vaccine. ASM Press, Washington, DC, pp 59–66
Briles DE, Hollingshead SK, Jonsdottir I (2008) Animal models of invasive pneumococcal disease. In: Siber GR, Klugman KP, Mäkelä PH (ed.), Pneumococcal vaccines: the impact of conjugate vaccine. ASM Press, Washington, DC, pp 47–58
Chiavolini D, Pozzi G, Ricci S (2008) Animal models of Streptococcus pneumoniae disease. Clin Microbiol Rev 21:666–685
Kadioglu A, Andrew PW (2005) Susceptibility and resistance to pneumococcal disease in mice. Brief Funct Genomic Proteomic 4(3):241–247
Blue CE, Mitchell TJ (2003) Contribution of a response regulator to the virulence of Streptococcus pneumoniae is strain dependent. Infect Immun 71:4405–4413
Kadioglu A, Weiser JN, Paton JC, Andrew PW (2008) The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease. Nat Rev Microbiol 6(4):288–301
Sandgren A, Albiger B, Orihuela CJ, Tuomanen E, Normark S, Henriques-Normark B (2005) Virulence in mice of pneumococcal clonal types with known invasive disease potential in humans. J Infect Dis 192(5):791–800
Nuermberger E (2005) Murine models of pneumococcal pneumonia and their applicability to the study of tissue-directed antimicrobials. Pharmacotherapy 25:134S–139S
Steinhoff MC (2007) Animal models for protein pneumococcal vaccine evaluation: a summary. Vaccine 25(13):2465–2470
Matrosovich M, Matrosovich T, Garten W, Klenk H-D Virol J 3(1):63
Zhang JR, Mostov KE, Lamm ME et al (2000) The polymeric immunoglobulin receptor translocates pneumococci across humannasopharyngeal epithelial cells. Cell. 102:827–37
Hentrich K, Löfling J, Pathak A et al (2016) Streptococcus pneumoniae senses a human-like sialic acid profile via the response regulator CiaR. Cell Host Microbe 20:307–317
Rayamajhi M, Redente EF, Condon TV et al (2011) Non-surgical intratracheal instillation of mice with analysis of lungs and lung draining lymph nodes by flow cytometry. J Vis Exp 51:e2702
Orihuela CJ, Gao G, Francis KP et al (2004) Tissue-specific contributions of pneumococcal virulence factors to pathogenesis. J Infect Dis 190:1661–1669
Iovino F, Engelen-Lee JY, Brouwer M et al (2017) pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion. J Exp Med 214:1619–1630
Iovino F, Thorsdottir S, Henriques-Normark B (2018) Receptor blockade: a novel approach to protect the brain from pneumococcal invasion. J Infect Dis 218(3):476–484
Shrestha S, Foxman B, Weinberger DM et al (2013) Identifying the interaction between influenza and pneumococcal pneumonia using incidence data. Sci Transl Med 5:191ra84
Iovino F, Seinen J, Henriques-Normark B et al (2016) How does Streptococcus pneumoniae invade the brain. Trends Microbiol 24:307–315
Orihuela CJ, Mahdavi J, Thornton J et al (2009) Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. J Clin Invest 119:1638–1646
Iovino F, Orihuela CJ, Moorlag HE et al (2013) Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis. PLoS One 8:e68408
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Iovino, F., Sender, V., Henriques-Normark, B. (2019). In Vivo Mouse Models to Study Pneumococcal Host Interaction and Invasive Pneumococcal Disease. In: Iovino, F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9199-0_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9198-3
Online ISBN: 978-1-4939-9199-0
eBook Packages: Springer Protocols