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Selection of Legionella Virulence-Related Traits by Environmental Protozoa

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

Predation by protozoa provides a strong selective pressure for Legionella to develop and maintain mechanisms conferring resistance to digestion and ability to replicate within both amoebae and mammalian macrophages. Here we describe how to isolate environmental protozoa that prey on virulent Legionella. These protists are extremely useful models to study the cellular mechanisms employed by Legionellae to survive and grow in its natural environment. We present here procedures that are available to study the interactions between environmental protozoa and Legionella and thus increase our current understanding of Legionella virulence and the infection process.

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Author information

Authors and Affiliations

  1. Departamento de Genética, Fisiología y Microbiología, Universidad Complutense de Madrid, Madrid, Spain

    Francisco Amaro

  2. Department of Microbiology, University of Chicago, Chicago, IL, USA

    Howard Shuman

Authors
  1. Francisco Amaro
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  2. Howard Shuman
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Corresponding author

Correspondence to Howard Shuman .

Editor information

Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Amaro, F., Shuman, H. (2019). Selection of Legionella Virulence-Related Traits by Environmental Protozoa. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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