Abstract
Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.
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Acknowledgements
This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant to N.K.V. and the Singapore Ministry of Education under its Singapore Ministry of Education (MOE) Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004). A.K. acknowledges Ph.D. fellowship provided by Lee Kong Chian School of Medicine, Nanyang Technological University Singapore.
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Fazil, M.H.U.T., Ong, S.T., Chalasani, M.L.S., Kizhakeyil, A., Verma, N.K. (2019). GapmeR-Mediated Gene Silencing in Motile T-Cells. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_9
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DOI: https://doi.org/10.1007/978-1-4939-9036-8_9
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