Abstract
Fourier-transform infrared spectroscopy (FTIR) is a powerful tool for examining the metal coordination of the side chain COO− groups of Glu and Asp on Ca2+-binding proteins in solution. The behavior of COO− symmetric stretch can be investigated by using protein samples in H2O solution. However, it is difficult to obtain information about the behavior of the COO− antisymmetric stretch in H2O solution, because the COO− antisymmetric stretching band overlaps with the amide II band. Therefore, to obtain reliable infrared spectra in the region of COO− antisymmetric stretch, exchangeable protons in the protein should be completely deuterated by incubating the apoprotein dissolved in D2O under mild heating conditions.
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Nara, M., Morii, H., Tanokura, M. (2019). Coordination to Divalent Cations by Calcium-Binding Proteins. In: Heizmann, C. (eds) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology, vol 1929. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9030-6_9
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DOI: https://doi.org/10.1007/978-1-4939-9030-6_9
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