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Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging

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Cancer Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1928))

Abstract

Altered metabolism is a hallmark of cancer, both resulting from and driving oncogenesis. The NAD and NADP redox couples play a key role in a large number of the metabolic pathways involved. In their reduced forms, NADH and NADPH, these molecules are intrinsically fluorescent. As the average time for fluorescence to be emitted following excitation by a laser pulse, the fluorescence lifetime, is exquisitely sensitive to changes in the local environment of the fluorophore, imaging the fluorescence lifetime of NADH and NADPH offers the potential for label-free monitoring of metabolic changes inside living tumors. Here, we describe the biological, photophysical, and methodological considerations required to establish fluorescence lifetime imaging (FLIM) of NAD(P)H as a routine method for profiling the metabolism of living cancer cells and tissues.

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Authors and Affiliations

  1. Research Department of Cell & Developmental Biology, University College London, London, UK

    Thomas S. Blacker, Michael D. E. Sewell, Gyorgy Szabadkai & Michael R. Duchen

  2. UCL Consortium for Mitochondrial Research, University College London, London, UK

    Thomas S. Blacker, Gyorgy Szabadkai & Michael R. Duchen

  3. Department of Physics & Astronomy, University College London, London, UK

    Thomas S. Blacker

  4. The Francis Crick Institute, London, UK

    Gyorgy Szabadkai

  5. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Gyorgy Szabadkai

Authors
  1. Thomas S. Blacker
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  2. Michael D. E. Sewell
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  3. Gyorgy Szabadkai
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  4. Michael R. Duchen
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Corresponding author

Correspondence to Thomas S. Blacker .

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Editors and Affiliations

  1. National Cancer Institute, North Bethesda, MD, USA

    Majda Haznadar

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Blacker, T.S., Sewell, M.D.E., Szabadkai, G., Duchen, M.R. (2019). Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging. In: Haznadar, M. (eds) Cancer Metabolism. Methods in Molecular Biology, vol 1928. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9027-6_19

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  • DOI: https://doi.org/10.1007/978-1-4939-9027-6_19

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