Abstract
Human pluripotent stem cells (hPSCs) have been well known for their ability to generate kidney cell types. We developed a protocol that utilizes a set of growth factors to give rise to kidney progenitors, which when differentiated further in a monolayer gives rise to podocyte precursors, mesangial cells, proximal and distal tubular epithelial cells, and collecting duct cells. This article describes in detail how to obtain each of these segment-specific kidney cell types from hPSCs. Once obtained as a homogenous population, these cells are invaluable for nephrotoxicity testing, for disease modeling, and in tissue engineering approaches such as 3D bioprinting and seeding on acellular matrices and scaffolds.
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References
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Acknowledgments
This work was supported by Berlin-Brandenburg School for Regenerative Therapies and the German Federal Ministry of Education and Research (VIP, FKZ 03V0396, and FKZ 031A303B).
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Hariharan, K., Reinke, P., Kurtz, A. (2019). Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_9
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DOI: https://doi.org/10.1007/978-1-4939-9021-4_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9020-7
Online ISBN: 978-1-4939-9021-4
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