Abstract
The chick embryo is a powerful model for experimental embryology due to its accessibility, sturdiness, and ease of manipulation. Here we describe protocols for analysis of protein-DNA and protein-protein interactions in tissues and cells isolated from the developing chick. These assays are aimed at the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, can be used for the delineation of gene regulatory circuits.
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References
Davidson E (2009) The regulatory genome: gene regulatory networks in development and evolution. Elsevier, Burlington, MA
Gilmour DS, Lis JT (1984) Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes. Proc Natl Acad Sci U S A 81(14):4275–4279
Orlando V (2000) Mapping chromosomal proteins in vivo by formaldehyde-crosslinked-chromatin immunoprecipitation. Trends Biochem Sci 25(3):99–104
Kaboord B, Perr M (2008) Isolation of proteins and protein complexes by immunoprecipitation. Methods Mol Biol 424:349–364. https://doi.org/10.1007/978-1-60327-064-9_27
Gammill LS, Krull CE (2011) Embryological and genetic manipulation of chick development. Methods Mol Biol 770:119–137. https://doi.org/10.1007/978-1-61779-210-6_5
Betancur P, Bronner-Fraser M, Sauka-Spengler T (2010) Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest. Proc Natl Acad Sci U S A 107(8):3570–3575
Huang C, Jacobson K (2010) Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation. BioTechniques 49(6):881–886. https://doi.org/10.2144/000113550
Soderberg O, Gullberg M, Jarvius M, Ridderstrale K, Leuchowius KJ, Jarvius J et al (2006) Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat Methods 3(12):995–1000. https://doi.org/10.1038/nmeth947
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Azambuja, A.P., Simoes-Costa, M. (2019). Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos. In: Pelegri, F. (eds) Vertebrate Embryogenesis. Methods in Molecular Biology, vol 1920. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9009-2_7
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DOI: https://doi.org/10.1007/978-1-4939-9009-2_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9008-5
Online ISBN: 978-1-4939-9009-2
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