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Analysis of Transcriptional Regulation in Bone Cells

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Book cover Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

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Abstract

Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.

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Authors and Affiliations

  1. South West Thames Regional Genetics Laboratory, St George’s Hospital, London, UK

    Huilin Jin

  2. Kennedy Institute of Rheumatology, London, UK

    Huilin Jin

  3. Department of Life Sciences, School of Sciences, European University Cyprus, Nicosia, Cyprus

    Antonia Sophocleous

  4. Rheumatic Diseases Unit, Centre for Genomics and Experimental Medicine, MRC IGMM, Western General Hospital, University of Edinburgh, Edinburgh, UK

    Asim Azfer & Stuart H. Ralston

Authors
  1. Huilin Jin
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  2. Antonia Sophocleous
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  3. Asim Azfer
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  4. Stuart H. Ralston
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Corresponding author

Correspondence to Huilin Jin .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Jin, H., Sophocleous, A., Azfer, A., Ralston, S.H. (2019). Analysis of Transcriptional Regulation in Bone Cells. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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