Skip to main content
SpringerLink
Log in
Book cover

Bone Research Protocols pp 451–465Cite as

Techniques for the Study of Apoptosis in Bone

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in bone metabolism and associated bone disease or loss of structural integrity. Here we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Wyllie AH, Kerr JF, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251–306

    Article  CAS  Google Scholar 

  2. Stevens HY, Reeve J, Noble BS (2000) Bcl-2, tissue transglutaminase and p53 protein expression in the apoptotic cascade in ribs of premature infants. J Anat 196:181–191

    Article  Google Scholar 

  3. Jilka RL, Weinstein RS, Bellido T, Roberson P, Parfitt AM, Manolagas SC (1999) Increased bone formation by prevention of osteoblast apoptosis with parathyroid hormone. J Clin Invest 104:439–446

    Article  CAS  Google Scholar 

  4. Kameda T, Ishikawa H, Tsutsui T (1995) Detection and characterization of apoptosis in osteoclasts in vitro. Biochem Biophys Res Commun 207:753–760

    Article  CAS  Google Scholar 

  5. Verborgt O, Gibson GJ, Schaffler MB (2000) Loss of osteocyte integrity in association with microdamage and bone remodeling after fatigue in vivo. J Bone Miner Res 15:60–67

    Article  CAS  Google Scholar 

  6. Kogianni G, Mann V, Noble BS (2008) Apoptotic bodies convey activity capable of initiating osteoclastogenesis and localized bone destruction. J Bone Miner Res 23:915–927

    Article  Google Scholar 

  7. Noble BS, Stevens H, Loveridge N, Reeve J (1997) Identification of apoptotic changes in osteocytes in normal and pathological human bone. Bone 20:273–282

    Article  CAS  Google Scholar 

  8. Wong SYP, Evans RA, Needs C, Dunstan C, Hills E, Garvan J (1987) The pathogenesis of osteoarthritis of the hip: evidence for primary osteocyte death. Clin Orthop Relat Res 214:305–312

    Google Scholar 

  9. Canalis E, Mazziotii G, Giustina A, Bilezikian J (2007) Glucocorticoid-induced osteoporosis: pathophysiology and therapy. Osteoporos Int 18:1319–1328

    Article  CAS  Google Scholar 

  10. Davis HM, Aref MW, Aguilar-Perez A, Pacheco-Costa R, Allen K, Valdez S, Herrera C, Atkinson EG, Mohammad A, Lopez D, Harris MA (2018) Cx43 overexpression in osteocytes prevents osteocyte apoptosis and preserves cortical bone quality in aging mice. JBMR Plus 2(4):206–216

    Article  CAS  Google Scholar 

  11. Yang Z, Klionsky DJ (2010) Eaten alive: a history of macroautophagy. Nat Cell Biol 12(9):814

    Article  CAS  Google Scholar 

  12. Florencio-Silva R, Sasso GR, Sasso-Cerri E, Simões MJ, Cerri PS (2018) Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats. Biomed Pharmacother 98:406–415

    Article  CAS  Google Scholar 

  13. Noble BS, Peet N, Stevens HY, Brabbs A, Mosley JR, Reilly GC et al (2003) Mechanical loading: biphasic osteocyte survival and targeting of osteoclasts for bone destruction in rat cortical bone. Am J Physiol Cell Physiol 284(4):C934–C943

    Article  CAS  Google Scholar 

  14. Zhang B, Hou R, Zou Z, Luo T, Zhang Y, Wang L, Wang B (2018) Mechanically induced autophagy is associated with ATP metabolism and cellular viability in osteocytes in vitro. Redox Biol 14:492–498

    Article  CAS  Google Scholar 

  15. Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555–556

    Article  CAS  Google Scholar 

  16. Oberhammer F, Wilson JW, Dive C et al (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J 12:3679–3684

    Article  CAS  Google Scholar 

  17. Slee EA, Adrain C, Martin SJ (1999) Serial killers: ordering caspase activation events in apoptosis. Cell Death Differ 6:1067–1074

    Article  CAS  Google Scholar 

  18. Yuan J, Shaham S, Ledoux S, Ellis HM, Horvitz HR (1993) The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 [beta]-converting enzyme. Cell 75:641–652

    Article  CAS  Google Scholar 

  19. Creagh E, Martin S (2001) Caspases: cellular demolition experts. Biochem Soc Trans 29:696–701

    Article  CAS  Google Scholar 

  20. Slee EA, Harte MT, Kluck RM et al (1999) Ordering the cytochrome c-initiated Caspase cascade: hierarchical activation of Caspases-2, À3, À6, À7, À8, and À10 in a Caspase-9-dependent manner. J Cell Biol 144:281–292

    Article  CAS  Google Scholar 

  21. Farquharson C, Whitehead C, Rennie S, Thorp B, Loveridge N (1992) Cell proliferation and enzyme activities associated with the development of avian tibial dyschondroplasia: an in situ biochemical study. Bone 13:59–67

    Article  CAS  Google Scholar 

  22. Ekert PG, Silke J, Vaux DL (1999) Caspase inhibitors. Cell Death Differ 6:1081–1086

    Article  CAS  Google Scholar 

  23. Mann V, Huber C, Kogianni G, Collins F, Noble B (2007) The antioxidant effect of estrogen and selective estrogen receptor modulators in the inhibition of osteocyte apoptosis in vitro. Bone 40:674–684

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Medicine and Dentistry, University of Plymouth, Plymouth, UK

    Val Mann

  2. School of Life Sciences, University of Westminster, London, UK

    Brendon Noble

Authors
  1. Val Mann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brendon Noble
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Mann, V., Noble, B. (2019). Techniques for the Study of Apoptosis in Bone. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_26

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_26

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation