Skip to main content
SpringerLink
Log in

Animal Models of Breast Cancer Bone Metastasis

  • Protocol
  • First Online:
Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

This chapter is designed to provide a comprehensive overview outlining the different in vivo models available for research into breast cancer bone metastasis. The main focus is to guide the researcher through the methodological processes required to establish and utilize these models within their own laboratory. These detailed methods are designed to enable the acquisition of accurate and meaningful results that can be used for publication and future translation into clinical benefit for women with breast cancer-induced bone metastasis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Coleman RE (1997) Skeletal complications of malignancy. Cancer 80:1588–1594

    Article  CAS  Google Scholar 

  2. Ottewell PD, O’Donnell L, Holen I (2015) Molecular alterations that drive breast cancer metastasis to bone. Bonekey Rep 4:643

    Article  CAS  Google Scholar 

  3. Cox TR, Rumney RMH, Schoof EM, Perryman L, Høye AM, Agrawal A, Bird D, Latif NA, Forrest H, Evans HR, Huggins ID, Lang G, Linding R, Gartland A, Erler JT (2015) The hypoxic cancer secretome induces pre-metastatic bone lesions through lysyl oxidase. Nature 522(7554):106–110

    Article  CAS  Google Scholar 

  4. Wright LE, Ottewell PD, Rucci N, Peyruchaud O, Pagnotti GM, Chiechi A, Buijs JT, Sterling JA (2016) Murine models of breast cancer bone metastasis. Bonekey Rep 5:804

    Article  CAS  Google Scholar 

  5. Holen I, Walker M, Nutter F, Fowles A, Evans CA, Eaton CL, Ottewell PD (2016) Oestrogen receptor positive breast cancer metastasis to bone: inhibition by targeting the bone microenvironment in vivo. Clin Exp Metastasis 33(3):211–224

    Article  CAS  Google Scholar 

  6. Ottewell PD, Deux B, Mönkkönen H, Cross S, Coleman RE, Clezardin P, Holen I (2008) Differential effect of doxorubicin and zoledronic acid on intraosseous versus extraosseous breast tumor growth in vivo. Clin Cancer Res 14(14):4658–4666

    Article  CAS  Google Scholar 

  7. Ottewell PD, Woodward JK, Lefley DV, Evans CA, Coleman RE, Holen I (2009) Anticancer mechanisms of doxorubicin and zoledronic acid in breast cancer tumor growth in bone. Mol Cancer Ther 8(10):2821–2832

    Article  CAS  Google Scholar 

  8. Withana NP, Blum G, Sameni M, Slaney C, Anbalagan A, Olive MB, Bidwell BN, Edgington L, Wang L, Moin K, Sloane BF, Anderson RL, Bogyo MS, Parker BS (2012) Cathepsin B inhibition limits bone metastasis in breast cancer. Cancer Res 72(5):1199–1209

    Article  CAS  Google Scholar 

  9. Bretschi M, Merz M, Komljenovic D, Berger MR, Semmler W, Bäuerle T (2011) Cilengitide inhibits metastatic bone colonization in a nude rat model. Oncol Rep 26(4):843–851

    CAS  PubMed  Google Scholar 

  10. Tulotta C, He S, van der Ent W, Chen L, Groenewoud A, Spaink HP, Snaar-Jagalska BE (2016) Imaging cancer angiogenesis and metastasis in a zebrafish embryo model. Adv Exp Med Biol 916:239–263

    Article  CAS  Google Scholar 

  11. Mercatali L, La Manna F, Groenewoud A, Casadei R, Recine F, Miserocchi G, Pieri F, Liverani C, Bongiovanni A, Spadazzi C, de Vita A, van der Pluijm G, Giorgini A, Biagini R, Amadori D, Ibrahim T, Snaar-Jagalska E (2016) Development of a patient-derived xenograft (PDX) of breast cancer bone metastasis in a zebrafish model. Int J Mol Sci 17(8):E1375. https://doi.org/10.3390/ijms17081375

    Article  CAS  PubMed  Google Scholar 

  12. Lee JH, Kim B, Jin WJ, Kim JW, Kim HH, Ha H, Lee ZH (2014) Trolox inhibits osteolytic bone metastasis of breast cancer through both PGE2-dependent and independent mechanisms. Biochem Pharmacol 91(1):51–60

    Article  CAS  Google Scholar 

  13. Kuperwasser C, Dessain S, Bierbaum BE, Garnet D, Sperandio K, Gauvin GP, Naber SP, Weinberg RA, Rosenblatt M (2005) A mouse model of human breast cancer metastasis to human bone. Cancer Res 65(14):6130–6138

    Article  CAS  Google Scholar 

  14. Holen I, Nutter F, Wilkinson JM, Evans CA, Avgoustou P, Ottewell PD (2015) Human breast cancer bone metastasis in vitro and in vivo: a novel 3D model system for studies of tumour cell-bone cell interactions. Clin Exp Metastasis 32(7):689–702

    Article  CAS  Google Scholar 

  15. Ottewell PD, Wang N, Brown HK, Reeves KJ, Fowles CA, Croucher PI, Eaton CL (2014) Holen I. Zoledronic acid has differential antitumor activity in the pre- and postmenopausal bone microenvironment in vivo. Clin Cancer Res 20(11):2922–2932

    Article  CAS  Google Scholar 

  16. Suva LJ, Washam C, Nicholas RW, Griffin RJ (2011) Bone metastasis: mechanisms and therapeutic opportunities. Nat Rev Endocrinol 7(4):208–218

    Article  CAS  Google Scholar 

  17. Nutter F, Holen I, Brown HK, Cross SS, Evans CA, Walker M, Coleman RE, Westbrook JA, Selby PJ, Brown JE, Ottewell PD (2014) Different molecular profiles are associated with breast cancer cell homing compared with colonisation of bone: evidence using a novel bone-seeking cell line. Endocr Relat Cancer 21(2):327–341

    Article  CAS  Google Scholar 

  18. Wetterwald A, van der Pluijm G, Que I, Sijmons B, Buijs J, Karperien M, Löwik CWGM, Gautschi E, Thalmann GN, Cecchini MG (2002) Optical imaging of cancer metastasis to bone marrow. Am J Pathol 160(3):1143–1153

    Article  Google Scholar 

  19. Pécheur I, Peyruchaud O, Serre CM, Guglielmi J, Voland C, Bourre F, Margue C, Cohen-Solal M, Buffet A, Kieffer N, Integrin CP (2002) alpha(v)beta3 expression confers on tumor cells a greater propensity to metastasize to bone. FASEB J 16(10):1266–1268

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oncology and Metabolism, Mellanby Centre for Bone Research, University of Sheffield, Sheffield, UK

    Claudia Tulotta & Penelope Ottewell

  2. Institute of Biology, Leiden University, Leiden, The Netherlands

    Arwin Groenewoud & B. Ewa Snaar-Jagalska

Authors
  1. Claudia Tulotta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Arwin Groenewoud
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Ewa Snaar-Jagalska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Penelope Ottewell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Penelope Ottewell .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Tulotta, C., Groenewoud, A., Snaar-Jagalska, B.E., Ottewell, P. (2019). Animal Models of Breast Cancer Bone Metastasis. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation