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Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

Protein-protein interactions provide a common mechanism for regulating protein functions and also serve as the fundamental step of many biochemical reactions. To accurately determine the involvement and function of protein-protein interactions, it is crucial to detect the interactions with the minimum number of artifacts. In this chapter, we report the method of bimolecular fluorescence complementation (BiFC) in tissue culture and developing tissues of Drosophila, which allows the visualization of subcellular localization of protein-protein interactions in living cells.

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Acknowledgments

We would like to thank Dr. Yaoting Deng for sharing her data on Hpo dimerization. This work was partly supported by the National Science Foundation.

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Authors and Affiliations

  1. Intercollege Graduate Degree Program in Molecular, Cellular and Integrative Biosciences, The Pennsylvania State University, University Park, PA, USA

    Yurika Matsui & Zhi-Chun Lai

  2. Department of Biology, The Pennsylvania State University, University Park, PA, USA

    Zhi-Chun Lai

  3. Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA

    Zhi-Chun Lai

Authors
  1. Yurika Matsui
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  2. Zhi-Chun Lai
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Corresponding author

Correspondence to Zhi-Chun Lai .

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Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Matsui, Y., Lai, ZC. (2019). Bimolecular Fluorescence Complementation (BiFC) in Tissue Culture and in Developing Tissues of Drosophila to Study Protein-Protein Interactions. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

  • eBook Packages: Springer Protocols

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