Abstract
Fermented foods produced using edible microorganisms have been consumed worldwide since ancient times. Microorganisms produce some healthy functional substances. Recently, many people have been attracted to the notion that d-body amino acids have healthy functional effects. d-body amino acids convert l-body amino acid proteolysis from a substrate such as foods during fermentation. This chapter presents a description of methods used for d-alanine assays in the solution as medium and AARase assays in lactic acid bacteria (LAB) cells for isolation of high AARase-containing strains using d-amino acid oxidase and lactic acid dehydrogenase via a NADH oxidoreduction system.
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Matsumoto, N., Kanauchi, M. (2019). Assaying d-Body Amino Acids as d-Alanine and Amino Acid Racemase (AARase) Activity Using NADH Oxidoreduction Enzymic System. In: Kanauchi, M. (eds) Lactic Acid Bacteria. Methods in Molecular Biology, vol 1887. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8907-2_3
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DOI: https://doi.org/10.1007/978-1-4939-8907-2_3
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8907-2
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