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The Cell Painting Assay as a Screening Tool for the Discovery of Bioactivities in New Chemical Matter

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Systems Chemical Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1888))

Abstract

Multiparametric phenotypic screening based on cellular morphology interrogates many biological pathways simultaneously and is therefore a valuable screening tool for the discovery of new biological activities. The cell painting assay stains various cellular features using six different dyes in one well. By automated image analysis, hundreds of parameters are calculated from the images which deliver a phenotypic profile of the cell. It has been shown that compounds with similar modes of action deliver similar phenotypic profiles. Using a reference set of compounds with known modes of action, it is possible to assign probable modes of action to new compounds and to discover compounds with potentially new modes of action.

Here we describe the cell painting assay as a screening tool using a hit identification workflow which has been implemented using open-source software.

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Authors and Affiliations

  1. Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Axel Pahl & Sonja Sievers

Authors
  1. Axel Pahl
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  2. Sonja Sievers
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Correspondence to Sonja Sievers .

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Editors and Affiliations

  1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Slava Ziegler

  2. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Herbert Waldmann

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Pahl, A., Sievers, S. (2019). The Cell Painting Assay as a Screening Tool for the Discovery of Bioactivities in New Chemical Matter. In: Ziegler, S., Waldmann, H. (eds) Systems Chemical Biology. Methods in Molecular Biology, vol 1888. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8891-4_6

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  • DOI: https://doi.org/10.1007/978-1-4939-8891-4_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8890-7

  • Online ISBN: 978-1-4939-8891-4

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