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Characterization of the “Autophagic Flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy

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Autophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1880))

Abstract

A method is described for the immunostaining of human prostate cancer biopsies for the autophagic marker LC3 and the lysosomal protein LAMP2A. Using this combination we can provide some information on autophagic flux, and specific patterns of staining have been recognized for normal prostate tissue and prostatic carcinomas.

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References

  1. http://www.wcrf.org/int/cancer-facts-figures/data-specific-cancers/prostate-cancer-statistics

  2. Hamdy FC et al (2016) 10-year outcomes after monitoring, surgery, or radiotherapy for localized prostate Cancer. N Engl J Med 375(15):1415–1424

    Article  Google Scholar 

  3. Kabeya Y, Mizushima N, Ueno T, Yamamoto A et al (2000) LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J 19(21):5720–5728

    Article  CAS  Google Scholar 

  4. Koukourakis MI, Kalamida D, Mitrakas A et al (2015) Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer. Biochem Biophys Res Commun 461(2):268–274

    Article  CAS  Google Scholar 

  5. Koukourakis MI, Kalamida D, Giatromanolaki A et al (2015) Autophagosome proteins LC3A, LC3B and LC3C have distinct subcellular distribution kinetics and expression in cancer cell lines. PLoS One 10(9):e0137675

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Radiotherapy/Oncology, Democritus University of Thrace, and University General Hospital of Alexandroupolis, Alexandroupolis, Greece

    D. Kalamida & M. I. Koukourakis

  2. Department of Pathology, Democritus University of Thrace, and University General Hospital of Alexandroupolis, Alexandroupolis, Greece

    A. Giatromanolaki

Authors
  1. D. Kalamida
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  2. A. Giatromanolaki
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  3. M. I. Koukourakis
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Corresponding author

Correspondence to M. I. Koukourakis .

Editor information

Editors and Affiliations

  1. Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis

  2. Signalling Programme, Babraham Institute, Cambridge, UK

    Oliver Florey

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Kalamida, D., Giatromanolaki, A., Koukourakis, M.I. (2019). Characterization of the “Autophagic Flux” in Prostate Cancer Tissue Biopsies by LC3A/LAMP2a Immunofluorescence and Confocal Microscopy. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_36

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  • DOI: https://doi.org/10.1007/978-1-4939-8873-0_36

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8872-3

  • Online ISBN: 978-1-4939-8873-0

  • eBook Packages: Springer Protocols

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