Abstract
Most ion channels and receptors are distributed in cell membranes and are known as membrane proteins. These membrane proteins are folded in the cell membrane and become functional proteins. Here, we demonstrate a method of reconstructing membrane proteins into liposome membranes which are commonly used as artificial cell membranes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Suzuki Y, Ogasawara T, Tanaka Y et al (2018) Functional G-protein-coupled receptor (GPCR) synthesis: the pharmacological analysis of human histamine H1 receptor (HRH1) synthesized by a wheat germ cell-free protein synthesis system combined with asolectin glycerosomes. Front Pharmacol 9:38. https://doi.org/10.3389/fphar.2018.00038
De Grip JW, Van Oostrum J, Bovee-Geurts PH (1998) Selective detergent-extraction from mixed detergent/lipid/protein micelles, using cyclodextrin inclusion compounds: a novel generic approach for the preparation of proteoliposomes. Biochem J 330(2):667–674. https://doi.org/10.1042/bj3300667
Rigaud JL, Levy D, Mosser G et al (1998) Detergent removal by non-polar polystyrene beads. Eur Biophys J 27(4):305–319. https://doi.org/10.1007/s002490050138
Levy D, Bluzat A, Seigneuret M et al (1990) A systematic study of liposome and proteoliposome reconstitution involving Bio-Bead-mediated Triton X-100 removal. Biochim Biophys Acta 1025(2):179–190. https://doi.org/10.1016/0005-2736(90)90096-7
Knol J, Sjollema K, Poolman B (1998) Detergent-mediated reconstitution of membrane proteins. Biochem 37(46):16,410–16,415. https://doi.org/10.1021/bi981596u
Timothy AC (2008) Adv. Topics—Membrane proteins, U.S.-Canada Winter School on Biomolecular Solid State NMR. http://web.mit.edu/fbml/winterschool2008/talks/Fri2a%20-%20Cross_membrane_proteins.pdf
Ratnala, VRP (2004). Ligand–protein interaction of histamine with its human histamine H1 receptor target: high yield expression and magic-angle spinning NMR studies. Doctoral dissertation, Thesis, University of Leiden, The Netherlands
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Suzuki, Y. (2018). Reconstruction of Protein/Liposome Complex. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 1868. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8802-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8802-0_8
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8801-3
Online ISBN: 978-1-4939-8802-0
eBook Packages: Springer Protocols