Abstract
Nutrient starvation or inhibition of cellular metabolism can induce cancer cell death. This can be measured by a variety of methods. We describe here four simple methods to measure cell death in culture by using microscopy, western blot, and flow cytometry. We also provide tools to differentiate between different forms of cell death like apoptosis and necrosis by using chemical inhibitors.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Villa E, Ricci JE (2016) How does metabolism affect cell death in cancer? FEBS J 283(2016):2653–2660
Caro-Maldonado A, Muñoz-Pinedo C (2011) Dying for Something to Eat: How Cells Respond to Starvation. Open Cell Signal J 3:42–51
Galluzzi L, Bravo-San Pedro JM, Vitale I et al (2015) Essential versus accessory aspects of cell death: Recommendations of the NCCD 2015. Cell Death Differ 22:58–73
Logue SE, Elgendy M, Martin SJ (2009) Expression, purification and use of recombinant annexin V for the detection of apoptotic cells. Nat Protoc 4:1383–1395
Leõn-Annicchiarico CL, Ramírez-Peinado S, Domínguez-Villanueva D et al (2015) ATF4 mediates necrosis induced by glucose deprivation and apoptosis induced by 2-deoxyglucose in the same cells. FEBS J 282:3647–3658
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Püschel, F., Muñoz-Pinedo, C. (2019). Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_12
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8769-6_12
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8768-9
Online ISBN: 978-1-4939-8769-6
eBook Packages: Springer Protocols