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TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE

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Protein Gel Detection and Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1853))

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Abstract

Polyacrylamide gel electrophoresis (PAGE) has become one of the most powerful and widely used separation techniques for complex biological samples, whose traditional detection methods include organic dye or silver staining. For simple, convenient, and ultrasensitive detection of proteins after PAGE, a novel enhanced photoluminescent (PL) imaging method was developed. Thioglycolic acid (TGA)-capped CdTe quantum dots (QDs) and the enhancer reagent tetramethylethylenediamine (TEMED) were introduced, achieving the direct detection of various proteins in native 1-DE, 2-DE and SDS-PAGE. Here we describe the general protocol of TEMED enhanced PL imaging by QDs, including materials, practical procedures, and some notes.

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Acknowledgment

J. Ouyang thanks the financial support by the National Nature Science Foundation of China (21475011 and 21675014) and National Grant of Basic Research Program of China (2011CB915504). N. Na thanks the financial support provided by the National Nature Science Foundation of China (214220503 and 21675015) and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. College of Chemistry, Beijing Normal University, Beijing, P. R. China

    Na Na & Jin Ouyang

Authors
  1. Na Na
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  2. Jin Ouyang
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Corresponding author

Correspondence to Jin Ouyang .

Editor information

Editors and Affiliations

  1. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    Biji T. Kurien

  2. Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

    R. Hal Scofield

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Na, N., Ouyang, J. (2018). TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots After PAGE. In: Kurien, B., Scofield, R. (eds) Protein Gel Detection and Imaging. Methods in Molecular Biology, vol 1853. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8745-0_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8745-0_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8744-3

  • Online ISBN: 978-1-4939-8745-0

  • eBook Packages: Springer Protocols

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