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Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-Producing Bacteria

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Microbial Carotenoids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1852))

Abstract

Carotenoids are naturally occurring yellow to red pigments with many biological activities including antioxidant, anticancer, anti-inflammatory, membrane stabilizers, and precursors for vitamin A. These biological activities are linked with many health benefits (e.g., anticarcinogenic activity, prevention of chronic diseases, etc.), which grew the interest of several industrial sectors especially in food, feed, nutraceuticals, cosmetics, and pharmaceutical industries. The production of natural carotenoids from microbial sources such as bacteria can help meet the growing global market of carotenoids estimated at $1.5 billion in 2014 and is expected to reach 1.8 billion in 2019. This chapter demonstrates, step-by-step, the development of a rapid and selective screening method for isolation and identification of carotenoid-producing microorganisms and their carotenoid analysis. This method involves three main procedures: UV treatment, sequencing analysis of 16S rRNA genes, and carotenoids analysis using rapid and effective HPLC-diode array-MS methods.

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Author information

Authors and Affiliations

  1. Food Science and Technology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt

    Dalal Asker

  2. Department of Materials Science and Engineering, University of Toronto, Toronto, ON, Canada

    Dalal Asker & Tarek S. Awad

  3. Life Science Research Center, College of Bioresource Sciences, Nihon University, Fujisawa, Japan

    Teruhiko Beppu & Kenji Ueda

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  1. Dalal Asker
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  2. Tarek S. Awad
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  3. Teruhiko Beppu
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  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, León, Spain

    Carlos Barreiro

  2. Department of Biotechnology, Crystal Pharma, A Subsidiary of Albany, Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

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Asker, D., Awad, T.S., Beppu, T., Ueda, K. (2018). Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-Producing Bacteria. In: Barreiro, C., Barredo, JL. (eds) Microbial Carotenoids. Methods in Molecular Biology, vol 1852. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8742-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8742-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8741-2

  • Online ISBN: 978-1-4939-8742-9

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