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Detection, Labeling, and Culture of Lung Stem and Progenitor Cells

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Book cover Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1842))

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Abstract

Identification, isolation, and clonal culture of stem cells is essential for understanding their proliferative and differentiation potential, and the cellular and molecular mechanisms that regulate their fate. Akin to development in vivo, the in vitro growth of adult lung epithelial stem cells requires support of mesenchymal-derived growth factors. In the adult mouse lung, epithelial stem/progenitor cells are defined by the phenotype CD45neg CD31neg EpCAMpos CD104pos CD24low, and mesenchymal cells are defined by the phenotype CD45neg CD31neg EpCAMneg Sca-1hi. Here we describe a method for primary cell isolation from the adult mouse lung, a flow cytometry strategy for fractionation of epithelial stem/progenitor cells and mesenchymal cells, and a three-dimensional epithelial colony-forming assay.

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Acknowledgments

This work was supported by grants from RMIT University and the Australian National Health and Medical Research Council (NHMRC).

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Authors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, VIC, Australia

    Ivan Bertoncello

  2. Lung and Regenerative Medicine Institutes, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA

    Gianni Carraro

  3. School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, Australia

    Jonathan L. McQualter

Authors
  1. Ivan Bertoncello
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  2. Gianni Carraro
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  3. Jonathan L. McQualter
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Corresponding author

Correspondence to Jonathan L. McQualter .

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Editors and Affiliations

  1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh

  2. Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, NJ, USA

    Pranela Rameshwar

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Bertoncello, I., Carraro, G., McQualter, J.L. (2018). Detection, Labeling, and Culture of Lung Stem and Progenitor Cells. In: Singh, S., Rameshwar, P. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 1842. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8697-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8697-2_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8696-5

  • Online ISBN: 978-1-4939-8697-2

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