Abstract
Scientific photography is an important and indispensable tool in plant tissue culture research: photographs should be taken throughout a project for documentation. The aim of photography in plant tissue culture should be to illustrate clearly the differentiation, growth, and developmental stages occurring in vitro. Poor-quality scientific photography in tissue culture research and professional reports results in poor documentation. If visual aspects of the tissue culture are not well documented or not well reproduced in the image, an important part of the research is missed, the resulting report is of limited scientific value, and the research results may not be reproducible. Simple methods for improving the results of photography of materials from plant tissue culture are described and discussed, along with the necessary photographic equipment, suitable backgrounds, the construction of photographic plates, and correct use of electronic files for images. Finally, ethical concerns about image manipulation are discussed.
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References
Ray S (2015) Scientific photography and applied imaging. CRC Press, Boca Raton, FL
Gaba V, Shavit D, Steinitz B et al (2004) Photography as a tool of research and documentation in plant tissue culture. In Vitro Cell Dev Biol Plant 40:536–541. https://doi.org/10.1079/IVP2004544
Gray DJ (2000) Photographic methods for plant cell and tissue culture. In: Trigiano RN, Gray DJ (eds) Plant tissue culture concepts and laboratory exercises, 2nd edn. CRC Press, Boca Raton, pp 55–60
Bracegirdle B (1994) Scientific PhotoMACROgraphy. (Royal Microscopical Society Handbooks). BIOS Scientific, Abingdon
Branston C (2013) Focus on close-up and macro photography. Focal Press, Abingdon
Sholik S, Eggers R (2000) Macro and close-up photography handbook. Amherst Media, Buffalo, NY
Evennett PJ (1989) Image recording. In: Lacey AJ (ed) Light microscopy in biology. A practical approach. Oxford University Press, Oxford, pp 61–102
Lacey AJ (1999) Light microscopy in biology a practical approach, 2nd edn. Oxford, Oxford University Press
Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3909
Sheen J, Hwang S, Niwa Y et al (1995) Green-fluorescent protein as a new vital marker in plant cells. Plant J 8:777–784. https://doi.org/10.1046/j.1365-313X.1995.08050777.x
Olofsdotter M (1993) Image processing: a non-destructive method for measuring growth in cell and tissue culture. Plant Cell Rep 12:216–219. https://doi.org/10.1007/BF00237057
Toonen MA, Hendriks T, Schmidt ED et al (1994) Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking. Planta 194:565–572. https://doi.org/10.1007/BF00714471
Quiroz-Figueroa FR, Rojas-Herrera R, Galaz-Avalos RM et al (2006) Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell Tissue Organ Cult 86:285–301. https://doi.org/10.1007/s11240-006-9139-6
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Fraser B (2004) Understanding digital raw capture. http://wwwimages.adobe.com/www.adobe.com/content/dam/Adobe/en/products/photoshop/pdfs/understanding_digitalrawcapture.pdf. Accessed 10 May 2017
Verhoeven GJJ (2010) It's all about the format--unleashing the power of RAW aerial photography. Int J Remote Sens 31:2009–2042. https://doi.org/10.1080/01431160902929271
Rouse D. http://digital-photography-school.com/get-your-white-balance-right-in-seconds-using-grey-card/. Accessed 10 May 2017
Stewart CN Jr (2014) Images and imagination: the role of figures in plant cell and molecular biology publications. Plant Cell Rep 33:829–830. https://doi.org/10.1007/s00299-014-1579-6
Springer Nature (2016) Research data policies. http://www.springernature.com/gp/group/data-policy/. Accessed 10 May 2017
Nature (undated) Data availability statements and data citations policy: guidance for authors. http://www.nature.com/authors/policies/data/data-availability-statements-data-citations.pdf. Accessed 10 May 2017
Newman A (2013) The art of detecting data and image manipulation. https://www.elsevier.com/editors-update/story/publishing-ethics/the-art-of-detecting-data-and-image-manipulation. Accessed 10 May 2017
Van Noorden R (2015) The image detective who roots out manuscript flaws. Nature. http://www.nature.com/news/the-image-detective-who-roots-out-manuscript-flaws-1.17749. Accessed 10 May 2017
Retraction Watch (2015). http://retractionwatch.com/2015/12/04/voinnet-retracts-highly-cited-paper-bringing-his-total-to-7/. Accessed 10 May 2017
Rossner M, Yamada KM (2004) What's in a picture? The temptation of image manipulation. J Cell Biol 166:11–15. https://doi.org/10.1083/jcb.200406019
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Contribution from the Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel, No. 570/17.
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Gaba, V., Tam, Y., Shavit, D., Steinitz, B. (2018). Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_5
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DOI: https://doi.org/10.1007/978-1-4939-8594-4_5
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