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Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC

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Book cover Planarian Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1774))

Abstract

SILAC (stable isotope labeling by amino acids in cell culture) proteomics enables the relative quantification of proteins in one or more biological samples by mass spectrometry. This technology is based on the metabolic incorporation of heavy isotope-labeled essential amino acids into nascent proteins in vivo. Here, we describe the preparation of SILAC protein samples from planarians, flatworms with high regenerative potential and tissue plasticity. Applications for SILAC proteomics of planarians include the analysis of protein abundances, protein–protein interactions and turnover rates during stem cell-based regeneration and tissue homeostasis.

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Acknowledgments

We thank S. Zanivan and M. Mann for sharing advice and SILAC mouse liver. This work was supported by the Max Planck Society and the Deutsche Forschungsgemeinschaft (SFB629, PAK479). The Bartscherer lab is part of the Cells-in-Motion Cluster of Excellence (EXC 1003-CiM) and is funded by the European Research Council (ERC-2016-StG 716894 IniReg). We thank J. Rink for helpful comments on the manuscript.

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Authors and Affiliations

  1. Novartis, Sandoz GmbH, Biochemiestr. 10, Kundl, 6250, Austria

    Alexander Böser

  2. Max Planck Research Group for Stem Cells and Regeneration, Max Planck Institute for Molecular Biomedicine, Münster, Germany

    Alexander Böser & Kerstin Bartscherer

  3. Medical Faculty, University of Münster, Münster, Germany

    Alexander Böser & Kerstin Bartscherer

  4. Bioanalytical Mass Spectrometry Facility, Max Planck Institute for Molecular Biomedicine, Münster, Germany

    Hannes C. A. Drexler

Authors
  1. Alexander Böser
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  2. Hannes C. A. Drexler
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  3. Kerstin Bartscherer
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Corresponding author

Correspondence to Kerstin Bartscherer .

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Editors and Affiliations

  1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

    Jochen C. Rink

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Böser, A., Drexler, H.C.A., Bartscherer, K. (2018). Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7802-1_24

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7800-7

  • Online ISBN: 978-1-4939-7802-1

  • eBook Packages: Springer Protocols

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