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Biomarkers for Alzheimer’s Disease Drug Development pp 125–137Cite as

Mass Spectrometry-Based Metabolomic Multiplatform for Alzheimer’s Disease Research

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1750))

Abstract

The integration of complementary analytical platforms has emerged as a suitable strategy to perform a comprehensive metabolomic characterization of complex biological systems. In this work, we describe the most important issues to be considered for the application of a mass spectrometry multiplatform in Alzheimer’s disease research, which combines direct analysis with electrospray and atmospheric pressure photoionization sources, as well as orthogonal hyphenated approaches based on reversed-phase ultrahigh-performance liquid chromatography and gas chromatography. These procedures have been optimized for the analysis of multiple biological samples from human patients and transgenic animal models, including blood serum, various brain regions (e.g., hippocampus, cortex, cerebellum, striatum, olfactory bulbs), and other peripheral organs (e.g., liver, kidney, spleen, thymus). It is noteworthy that the metabolomic pipeline here detailed has demonstrated a great potential for the investigation of metabolic perturbations underlying Alzheimer’s disease pathogenesis.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain

    Raúl González-Domínguez, Álvaro González-Domínguez, Ana Sayago & Ángeles Fernández-Recamales

  2. International Campus of Excellence CeiA3, University of Huelva, Huelva, Spain

    Raúl González-Domínguez, Álvaro González-Domínguez, Ana Sayago & Ángeles Fernández-Recamales

Authors
  1. Raúl González-Domínguez
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  2. Álvaro González-Domínguez
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  3. Ana Sayago
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  4. Ángeles Fernández-Recamales
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Corresponding author

Correspondence to Raúl González-Domínguez .

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Editors and Affiliations

  1. Department of Psychiatry and Psychotherapy, Ludwig-Maximilians-Universität München, Munich, Germany

    Robert Perneczky

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González-Domínguez, R., González-Domínguez, Á., Sayago, A., Fernández-Recamales, Á. (2018). Mass Spectrometry-Based Metabolomic Multiplatform for Alzheimer’s Disease Research. In: Perneczky, R. (eds) Biomarkers for Alzheimer’s Disease Drug Development. Methods in Molecular Biology, vol 1750. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7704-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7704-8_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7703-1

  • Online ISBN: 978-1-4939-7704-8

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