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Sertoli Cells pp 103–112Cite as

A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1748))

Abstract

An emerging body of evidences based on in vitro studies indicate that mitophagy (selective autophagic clearance of damaged mitochondria) is a prosurvival mechanism, specifically under exposure to various stressors. Sertoli cells (SCs) play essential roles in maintenance of spermatogenesis via paracrine interactions with germ cells and other somatic cells in the testis; however, studies investigating mitophagy in SCs are still very few. In this chapter, we give a brief review of mechanisms and detection methods of mitophagy in SCs based on our recent publications on animal models of ethanol toxicity and current literature. In addition, we provide a method for induction and ultrastructural identification of mitophagy in SCs of adult Wistar rats using a single intraperitoneal injection (5 g/kg) of ethanol. Proper understanding of mitophagy features and mechanisms in SCs may have therapeutic implications for infertility associated with alcoholism and other diseases characterized by mitochondrial dysfunction.

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Acknowledgments

This manuscript is partially supported by Grant-in-Aid for Scientific Research (Kakenhi) (No., 16K11073) from The Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Prof. Yoshinori Otsuki for critical advice and support of this study.

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Authors and Affiliations

  1. Division of Life Sciences, Department of Anatomy and Cell Biology, Osaka Medical College, Osaka, Japan

    Nabil Eid, Yuko Ito, Akio Horibe, Hitomi Hamaoka & Yoichi Kondo

Authors
  1. Nabil Eid
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  2. Yuko Ito
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  3. Akio Horibe
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  4. Hitomi Hamaoka
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  5. Yoichi Kondo
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Corresponding author

Correspondence to Nabil Eid .

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Editors and Affiliations

  1. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Marco G. Alves

  2. Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal

    Pedro F. Oliveira

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Eid, N., Ito, Y., Horibe, A., Hamaoka, H., Kondo, Y. (2018). A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells. In: Alves, M., Oliveira, P. (eds) Sertoli Cells. Methods in Molecular Biology, vol 1748. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7698-0_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7698-0_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7697-3

  • Online ISBN: 978-1-4939-7698-0

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