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Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols

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Book cover Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1747))

Abstract

Nitric oxide (NO) is a small diatomic molecule that regulates multiple physiological processes in animals, plants, and microorganisms. In animals, it is involved in vasodilation and neurotransmission and is present in exhaled breath. In plants, it regulates both plant immune function and numerous developmental programs. The high reactivity and short half-life of NO and cross-reactivity of its various derivatives make its quantification difficult. Different methods based on calorimetric, fluorometric, and chemiluminescent detection of NO and its derivatives are available, but all of them have significant limitations. Here we describe a method for the chemiluminescence-based quantification of NO using ozone-chemiluminescence technology in plants. This approach provides a sensitive, robust, and flexible approach for determining the levels of NO and its signaling products, protein S-nitrosothiols.

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Author information

Authors and Affiliations

  1. Department of Agriculture, Abdul Wali Khan University, Mardan, Pakistan

    Adil Hussain

  2. School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea

    Byung-Wook Yun

  3. Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Gary J. Loake

Authors
  1. Adil Hussain
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  2. Byung-Wook Yun
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  3. Gary J. Loake
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Corresponding author

Correspondence to Gary J. Loake .

Editor information

Editors and Affiliations

  1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel

  2. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Christian Lindermayr

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Hussain, A., Yun, BW., Loake, G.J. (2018). Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols. In: Mengel, A., Lindermayr, C. (eds) Nitric Oxide. Methods in Molecular Biology, vol 1747. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7695-9_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7695-9_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7694-2

  • Online ISBN: 978-1-4939-7695-9

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