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Preclinical MRI pp 423–439Cite as

Anesthesia and Monitoring of Animals During MRI Studies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1718))

Abstract

The use of imaging represents a major impact on the refinement and the reduction of in vivo studies in animal models, in particular for allowing longitudinal monitoring of the onset and the progression of disease within the same animal, and studying the biological effects of drug candidate and their therapeutic effectiveness. But the use of imaging procedures can affect animal physiology, and the need to anesthetize the animals for imaging entails potential health risks. During anesthesia, there is an inevitable autonomic nervous system depression which induces cardiovascular depression, respiratory depression, and hypothermia. Also other procedures associated with imaging such as animal preparation (e.g., fasting, premedication), blood sampling, and dosage/contrast agent injections can also affect physiology and animal welfare. All these factors are likely to have confounding effect on the outcome of the imaging studies and pose important concerns regarding the animal’s well-being, particularly when imaging immune deprived animals or diseased animals. We will discuss these challenges and considerations during imaging to maximize efficacious data while promoting animal welfare.

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Author information

Authors and Affiliations

  1. Centre for Trauma Sciences, Blizard Institute, Queen Mary University of London, London, UK

    Jordi L. Tremoleda

  2. Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, UK

    Sven Macholl & Jane K. Sosabowski

Authors
  1. Jordi L. Tremoleda
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  2. Sven Macholl
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  3. Jane K. Sosabowski
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Corresponding author

Correspondence to Jordi L. Tremoleda .

Editor information

Editors and Affiliations

  1. BIONAND, Andalusian Centre for Nanomedicine and Biotechnology, Junta de Andalucía, Universidad de Málaga, Málaga, Spain

    María Luisa García Martín

  2. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/UAM, Madrid, Spain

    Pilar López Larrubia

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Tremoleda, J.L., Macholl, S., Sosabowski, J.K. (2018). Anesthesia and Monitoring of Animals During MRI Studies. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_25

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  • DOI: https://doi.org/10.1007/978-1-4939-7531-0_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7530-3

  • Online ISBN: 978-1-4939-7531-0

  • eBook Packages: Springer Protocols

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