Abstract
Emerging microtechnologies are aimed at developing a microsystem with densely packed array structure, i.e., an array with a femtoliter reaction chamber, for highly sensitive and quantitative biological assays. Here, we describe a novel femtoliter chamber array system (arrayed lipid bilayer chambers, ALBiC) that contains approximately a million femtoliter chambers, each sealed with a phospholipid bilayer membrane with extremely high efficiency (>90%). This novel platform enables detection of membrane transporter activity at the single-molecule level and thus expands the applicability of femtoliter chamber arrays to highly sensitive assays of transporters.
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Acknowledgment
This work was supported by a Grant-in-Aid for Scientific Research No. 15H05591 and 15H01312 to R.W. from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by a Precursory Research for Embryonic Science (PRESTO) grant to R.W. from the Japan Science and Technology Agency.
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Watanabe, R., Soga, N., Ohdate, Sy., Noji, H. (2018). Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem. In: Yamaguchi, A., Nishino, K. (eds) Bacterial Multidrug Exporters. Methods in Molecular Biology, vol 1700. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7454-2_17
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DOI: https://doi.org/10.1007/978-1-4939-7454-2_17
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