Abstract
MLST is a molecular typing technique that involves the identification and clustering of bacterial isolates based on the partial sequence analysis of multiple housekeeping genes (generally seven) which are distributed across the length of the genome of the organism. The Cronobacter whole genus MLST scheme can be successfully used for an accurate species level identification and classification of this complex genus.
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References
Joseph S, Forsythe S (2011) Predominance of Cronobacter sakazakii sequence type 4 in neonatal infections. Emerg Infect Dis 17:1713–1715
Iversen C, Mullane N, McCardell B et al (2008) Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov.,comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int J Syst Evol Microbiol 58:1442–1447
Joseph S, Cetinkaya E, Drahovska H et al (2012) Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients. Int J Syst Evol Microbiol 62:1277–1283
Jackson EE, Forsythe SJ (2016) Comparative study of Cronobacter identification according to phenotyping methods. BMC Microbiol 16:146
Baldwin A, Loughlin M, Caubilla-Barron J et al (2009) Multilocus sequence typing of Cronobacter sakazakii and Cronobacter malonaticus reveals stable clonal structures with clinical significance which do not correlate with biotypes. BMC Microbiol 9:223
Joseph S, Sonbol H, Hariri S et al (2012) Diversity of the Cronobacter genus as revealed by multilocus sequence typing. J Clin Microbiol 50:3031–3039
Hariri S, Joseph S, Forsythe SJ (2013) Cronobacter sakazakii ST4 strains and neonatal meningitis, US. Emerg Infect Dis 19:175–177
Joseph S, Forsythe SJ (2012) Insights into the emergent bacterial pathogen Cronobacter spp., generated by multilocus sequence typing and analysis. Front Food Microbiol 23:397
Forsythe SJ, Dickins B, Jolley KA (2014) Cronobacter, the emergent bacterial pathogen Enterobacter sakazakii comes of age; MLST and whole genome sequence analysis. BMC Genomics 15:1121
Goujon M, McWilliam H, Li W et al (2010) A new bioinformatics analysis tools framework at EMBL–EBI. Nucleic Acids Res 38(Suppl 2):W695–W699
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Joseph, S., Forsythe, S. (2017). Multilocus Sequence Typing (MLST) for Cronobacter spp.. In: Bishop-Lilly, K. (eds) Diagnostic Bacteriology. Methods in Molecular Biology, vol 1616. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7037-7_16
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DOI: https://doi.org/10.1007/978-1-4939-7037-7_16
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