Abstract
Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Reubinoff BE, Pera MF, Vajta G, Trounson A (2002) Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Hum Reprod 16:2187–2194
Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H (1998) Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51:53–58
Crook JM, Peura TT, Kravets L et al (2007) The generation of six clinical grade human embryonic stem cell lines. Cell Stem Cell 1:490–494
Rall WF (2003) Avoidance of microbial cross-contamination of cryopreserved gametes, embryos, cells and tissues during storage in liquid nitrogen. The Embryologists’ 6:4–15
Crook JM and Kravets L (2005) Cell preservation method. Patent International Publication Number WO2005/118785 A1
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Crook, J.M., Tomaskovic-Crook, E., Ludwig, T.E. (2017). Cryobanking Pluripotent Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6921-0_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6919-7
Online ISBN: 978-1-4939-6921-0
eBook Packages: Springer Protocols