Abstract
The unique properties of vesicular stomatitis virus (VSV) make it a promising vaccine platform. With the advent of plasmid-based approaches to generate recombinant VSV viruses that express glycoproteins of other viruses, researchers now have the means to generate vaccine candidates targeting a variety of human pathogens. This chapter gives a general overview of the workings of VSV as a vaccine platform and provides a detailed protocol for the generation of recombinant VSV from plasmids.
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Acknowledgements
The authors acknowledge funding through NIH R21 AI121933 and NIH RO1 to JHC. We also acknowledge support through the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award UC6AI058618.
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Ruedas, J.B., Connor, J.H. (2017). Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms. In: Ferran, M., Skuse, G. (eds) Recombinant Virus Vaccines. Methods in Molecular Biology, vol 1581. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6869-5_12
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DOI: https://doi.org/10.1007/978-1-4939-6869-5_12
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