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ERK Signaling pp 289–301Cite as

Probing Chromatin Modifications in Response to ERK Signaling

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1487))

Abstract

Chromatin immunoprecipitation (ChIP) is a technique used to determine the association of proteins or histone modifications with chromatin regions in living cells or tissues, and is used extensively in the chromatin biology field to study transcriptional and epigenetic mechanisms. Increasing evidence points to an epigenetic coordination of signaling cascades, such as ERK, that regulate key processes in development and disease, revealing novel principles of gene regulation. Here we describe a detailed protocol for performing chromatin immunoprecipitation followed by qPCR (ChIP-qPCR) for probing histone modifications regulated by ERK signaling in mouse ESCs.

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Acknowledgments

Work in the W.-W.T. lab is supported by research fundings from the Singapore National Research Foundation Fellowship as well as the Biomedical Research Council, Agency for Science, Technology and Research.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Pharmacology, Howard Hughes Medical Institute, New York University School of Medicine, New York, NY, 10016, USA

    Ozgur Oksuz

  2. Institute of Molecular and Cell Biology (IMCB), A*STAR (Agency for Science, Technology, and Research), 61 Biopolis Drive, Proteos, Singapore, 138673, Singapore

    Wee-Wei Tee

  3. Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore

    Wee-Wei Tee

Authors
  1. Ozgur Oksuz
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  2. Wee-Wei Tee
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Corresponding author

Correspondence to Wee-Wei Tee .

Editor information

Editors and Affiliations

  1. Institut de Biologia Molecular de Barcelona-CSIC, Parc Científic de Barcelona, Barcelona, Spain

    Gerardo Jimenez

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Oksuz, O., Tee, WW. (2017). Probing Chromatin Modifications in Response to ERK Signaling. In: Jimenez, G. (eds) ERK Signaling. Methods in Molecular Biology, vol 1487. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6424-6_22

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  • DOI: https://doi.org/10.1007/978-1-4939-6424-6_22

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6422-2

  • Online ISBN: 978-1-4939-6424-6

  • eBook Packages: Springer Protocols

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