Abstract
With the rapid advances in prediction tools for discovery of new promoters and their cis-elements, there is a need to improve plant expression methodologies in order to facilitate a high-throughput functional validation of these promoters in planta. The promoter-reporter analysis is an indispensible approach for characterization of plant promoters. It requires the design of complex plant expression vectors, which can be challenging. Here, we describe the use of a plant grammar implemented in GenoCAD that will allow the users to quickly design constructs for promoter analysis experiments but also for other in planta functional studies. The GenoCAD plant grammar includes a library of plant biological parts organized in structural categories to facilitate their use and management and a set of rules that guides the process of assembling these biological parts into large constructs.
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Acknowledgments
This work was supported by NSF Awards 1241328, the Slovenian Research Agency Program P4-0165, and Project N4-0026.
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Coll, A., Wilson, M.L., Gruden, K., Peccoud, J. (2016). GenoCAD Plant Grammar to Design Plant Expression Vectors for Promoter Analysis. In: Hehl, R. (eds) Plant Synthetic Promoters. Methods in Molecular Biology, vol 1482. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6396-6_14
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DOI: https://doi.org/10.1007/978-1-4939-6396-6_14
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