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Thromboembolic Model of Cerebral Ischemia and Reperfusion in Mice

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Injury Models of the Central Nervous System

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1462))

Abstract

Ischemic stroke is the fourth leading cause of death in the USA and a prominent cause of death globally. Besides thrombolytic therapy used in a small subset of patients, no alternative therapeutic strategy has been shown to improve the outcome of stroke patients. Preclinical models of ischemic stroke are an essential tool for investigating pathogenic processes that happen after the ischemic insult, as well as to screen for candidate therapeutic interventions. There are several models of rodent ischemic stroke including mechanical occlusion, thromboembolic stroke, and photothrombotic stroke. However, models that permit studying stroke in the context of thrombolytic therapy, such as thromboembolic models, are becoming of increasing interest to the research community. In this chapter, we describe a thromboembolic model of ischemic stroke with and without tissue-plasminogen activator-induced reperfusion. We describe protocols for microemboli preparation, surgical procedure, and post-stroke assessment of animals.

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Acknowledgements

This work is funded by a Department of Veterans Affairs Merit Award (RX00114) and the NIH (P20GM109040) to S.T. and an American Heart Association Pre-doctoral Fellowship Award (15PRE25250009) to A.A.

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA

    Ali Alawieh, Wenxue Wang & Aarti Narang

  2. Neuroscience Institute, Medical University of South Carolina, Charleston, SC, 29425, USA

    Ali Alawieh, Wenxue Wang, Aarti Narang & Stephen Tomlinson Ph.D.

  3. Department of Microbiology and Immunology, Medical University of South Carolina, 173 Ashley Avenue, BSB 204 MSC 504, Charleston, SC, 29425, USA

    Stephen Tomlinson Ph.D.

  4. Ralph H. Johnson Veteran Affairs Medical Center, Charleston, SC, USA

    Stephen Tomlinson Ph.D.

Authors
  1. Ali Alawieh
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  2. Wenxue Wang
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  3. Aarti Narang
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  4. Stephen Tomlinson Ph.D.
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Corresponding author

Correspondence to Stephen Tomlinson Ph.D. .

Editor information

Editors and Affiliations

  1. Banyan Biomarkers, Inc., Alachua, Florida, USA

    Firas H. Kobeissy

  2. Safar Center for Resuscitation Research, University of Pittsburgh Safar Center for Resuscitation Research, Pittsburgh, Pennsylvania, USA

    C. Edward Dixon

  3. Banyan Biomarkers, Inc. , Alachua, Florida, USA

    Ronald L. Hayes

  4. Banyan Biomarkers, Inc., Alachua, Florida, USA

    Stefania Mondello

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Alawieh, A., Wang, W., Narang, A., Tomlinson, S. (2016). Thromboembolic Model of Cerebral Ischemia and Reperfusion in Mice. In: Kobeissy, F., Dixon, C., Hayes, R., Mondello, S. (eds) Injury Models of the Central Nervous System. Methods in Molecular Biology, vol 1462. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3816-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3816-2_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3814-8

  • Online ISBN: 978-1-4939-3816-2

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