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Sampling of Riverine or Marine Bacterial Communities in Remote Locations: From Field to Publication

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Marine Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1452))

Abstract

This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Illumina MiSeq amplicon data and analyze it in the R environment.

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Author information

Authors and Affiliations

  1. Centre for Ecology and Hydrology, Maclean Building, Benson Lane, Crowmarsh Gifford, Wallingford, OX10 8BB, UK

    Katja Lehmann

Authors
  1. Katja Lehmann
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Corresponding author

Correspondence to Katja Lehmann .

Editor information

Editors and Affiliations

  1. Department of Marine Sciences, University of Gothenburg, Gothenburg, Sweden

    Sarah J. Bourlat

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© 2016 Springer Science+Business Media New York

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Lehmann, K. (2016). Sampling of Riverine or Marine Bacterial Communities in Remote Locations: From Field to Publication. In: Bourlat, S. (eds) Marine Genomics. Methods in Molecular Biology, vol 1452. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3774-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3774-5_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3772-1

  • Online ISBN: 978-1-4939-3774-5

  • eBook Packages: Springer Protocols

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