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In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

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In Vivo Fluorescence Imaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1444))

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Abstract

Near-infrared fluorescence (NIRF) imaging is an emerging imaging technique for studying diseases at the molecular level. Optical imaging with a near-infrared emitting fluorophore for targeting tumor angiogenesis offers a noninvasive method for early tumor detection and efficient monitoring of tumor response to anti-angiogenesis therapy. CD13 receptor, a zinc-dependent membrane-bound ectopeptidase, plays important roles in regulating tumor angiogenesis and the growth of new blood vessels. In this chapter, we use CD13 receptor as an example to demonstrate how to construct CD13-specific NGR-containing peptides via bioorthogonal click chemistry for visualizing and quantifying the CD13 receptor expression in vivo by means of NIRF optical imaging.

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Acknowledgments

This work was supported by the American Cancer Society (#IRG-58-007-51), the Robert E. and May R. Wright Foundation, and the USC Department of Radiology.

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Authors and Affiliations

  1. Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, 2250 Alcazar Street, CSC 103, Los Angeles, CA, 90033-9061, USA

    Rui Huang, Peter S. Conti & Kai Chen

Authors
  1. Rui Huang
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  2. Peter S. Conti
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  3. Kai Chen
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Corresponding author

Correspondence to Kai Chen .

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Editors and Affiliations

  1. Univ of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Mingfeng Bai

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Huang, R., Conti, P.S., Chen, K. (2016). In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes. In: Bai, M. (eds) In Vivo Fluorescence Imaging. Methods in Molecular Biology, vol 1444. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3721-9_8

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  • DOI: https://doi.org/10.1007/978-1-4939-3721-9_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3719-6

  • Online ISBN: 978-1-4939-3721-9

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