Abstract
Bioinformatic sequence analysis allows the functional characterization of newly sequenced proteins. Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. The current omics era has enabled the exponential growth of the sequenced NRPS, and it is important to characterize the final product of these synthetases. Here, how to achieve the prediction of substrates which bind to adenylation domains in NRPS with NRPSsp (www.nrpssp.com) bioinformatic tool is described.
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Acknowledgments
This work was supported by Agencia de Inversiones y Servicios de Castilla y León (record CCTT/10/LE/0001) and by Juan de la Cierva programme (JCI-2009-05444) of the Ministry of Science and Innovation (Spain).
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Prieto, C. (2016). Characterization of Nonribosomal Peptide Synthetases with NRPSsp. In: Evans, B. (eds) Nonribosomal Peptide and Polyketide Biosynthesis. Methods in Molecular Biology, vol 1401. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3375-4_17
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DOI: https://doi.org/10.1007/978-1-4939-3375-4_17
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3375-4
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