Abstract
Circadian clocks drive 24 h biological rhythms to optimize physiology and development in response to the rotation of the planet. In plants, photosynthesis is modulated by the circadian clock and contributes to daily rhythms in cellular metabolism. In addition to light and temperature, sugar produced from photosynthesis acts as a zeitgeber to contribute to setting of the plant circadian clock. Here, we describe methods to manipulate photosynthetic output and sugar availability in Arabidopsis seedlings. These protocols have been applied to investigate the effects on the Arabidopsis circadian network, but are easily adaptable to other processes in plants.
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References
Woelfle MA, Ouyang Y, Phanvijhitsiri K, Johnson CH (2004) The adaptive value of circadian clocks : an experimental assessment in Cyanobacteria. Curr Biol 14:1481–1486
Dodd AN, Salathia N, Hall A et al (2005) Plant circadian clocks increase photosynthesis, growth, survival, and competitive advantage. Science 309:630–633
Johnson CH (1992) Phase response curves: what can they tell us about circadian clocks? In: Hiroshige T, Honma K (eds) Circadian clocks from cell to Human. Hokkaido Univ. Press, pp 209–249
Haydon MJ, Hearn TJ, Bell LJ et al (2013) Metabolic regulation of circadian clocks. Semin Cell Dev Biol 24:414–421
Rensing L, Ruoff P (2002) Temperature effect on entrainment, phase shifting, and amplitude of circadian clocks and its molecular bases. Chronobiol Int 19:807–864
Damiola F, Le Minh N, Preitner N et al (2000) Restricted feeding uncouples circadian oscillators in peripheral tissues from the central pacemaker in the suprachiasmatic nucleus. Genes Dev 14:2950–2961
Stokkan KA, Yamazaki S, Tei H et al (2001) Entrainment of the circadian clock in the liver by feeding. Science 291:490–493
Gutiérrez RA, Stokes TL, Thum K et al (2008) Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc Natl Acad Sci U S A 105:4939–4944
Haydon MJ, Mielzcarek O, Robertson FC et al (2013) Photosynthetic entrainment of the Arabidopsis circadian clock. Nature 502:689–692
Harmer SL, Hogenesch JB, Straume M et al (2000) Orchestrated transcription of key pathways in Arabidopsis by the circadian clock. Science 290:2110–2113
Noordally ZB, Ishii K, Atkins KA et al (2013) Circadian control of chloroplast transcription by a nuclear-encoded timing signal. Science 339:1316–1319
Lu Y, Gehan JP, Sharkey TD (2005) Daylength and circadian effects on starch degradation and maltose metabolism. Plant Physiol 138:2280–2291
Bläsing OE, Gibon Y, Günther M et al (2005) Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in Arabidopsis. Plant Cell 17:3257–3281
Millar AJ, Short SR, Hiratsuka K et al (1992) Firefly luciferase as a reporter of regulated gene expression in higher plants. Plant Mol Biol Report 10:324–337
Knight H, Thomson AJW, McWatters HG (2008) SENSITIVE TO FREEZING6 integrates cellular and environmental inputs to the plant circadian clock. Plant Physiol 148:293–303
Dalchau N, Baek SJ, Briggs HM et al (2011) The circadian oscillator gene GIGANTEA mediates a long-term response of the Arabidopsis thaliana circadian clock to sucrose. Proc Natl Acad Sci U S A 108:5104–5109
James AB, Monreal JA, Nimmo GA et al (2008) The circadian clock in Arabidopsis roots is a simplified slave version of the clock in shoots. Science 322:1832–1835
Kircher S, Schopfer P (2012) Photosynthetic sucrose acts as cotyledon-derived long-distance signal to control root growth during early seedling development in Arabidopsis. Proc Natl Acad Sci U S A 109:11217–11221
Aschoff J (1979) Circadian rhythms: influences of internal and external factors on the period measured in constant conditions. Z Tierpsychol 49:225–249
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Haydon, M.J., Webb, A.A.R. (2016). Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock. In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_12
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DOI: https://doi.org/10.1007/978-1-4939-3356-3_12
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3356-3
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