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Nanostructured Capsules for Cartilage Tissue Engineering

  • Protocol
Book cover Cartilage Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1340))

Abstract

Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(l-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(l-lactic acid) microparticles for cartilage tissue engineering.

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Acknowledgements

This work was financially supported by the Portuguese Foundation for Science and Technology (FCT) through the project PTDC/CTM-Bio/1814/2012 and Ph.D. grant with the reference no. SFRH/BD/69529/2010, co-financed by the Operational Human Potential Program (POPH), developed under the scope of the National Strategic Reference Framework (QREN) from the European Social Fund (FSE). The authors also acknowledge the financial support from Seventh Framework Programme of European Union (FP7/2007–2013) under grant agreement number REGPOT-CT2012-316331-POLARIS.

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Authors and Affiliations

  1. 3B’ s Research Group—Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4805-017 Barco, Guimarães, Portugal

    Clara R. Correia, Rui L. Reis & João F. Mano

  2. ICVS/3B’s—PT Government Associate Laboratory, Guimarães, Braga, Portugal

    Clara R. Correia, Rui L. Reis & João F. Mano

Authors
  1. Clara R. Correia
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  2. Rui L. Reis
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  3. João F. Mano
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Corresponding author

Correspondence to João F. Mano .

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Editors and Affiliations

  1. Swinburne University of Technology, Faculty of Science, Engineering and Technology, Hawthorn, Melbourne, Victoria, Australia

    Pauline M. Doran

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Correia, C.R., Reis, R.L., Mano, J.F. (2015). Nanostructured Capsules for Cartilage Tissue Engineering. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2938-2_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2937-5

  • Online ISBN: 978-1-4939-2938-2

  • eBook Packages: Springer Protocols

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