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Plant Gene Silencing pp 25–41Cite as

Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1287))

Abstract

The rapid progress in genome sequencing and transcriptome analysis in model and crop plants has made possible the identification of a vast number of genes potentially associated with economically important complex traits. The ultimate goal is to assign functions to these genes by using forward and reverse genetic screens. Plant viruses have been developed for virus-induced gene silencing (VIGS) to generate rapid gene knockdown phenotypes in numerous plant species. To fulfill its potential for high-throughput phenomics, it is of prime importance to ensure that parameters conditioning the VIGS response, i.e., plant–virus interactions and associated loss-of-function screens, are “fit for purpose” and optimized to unequivocally conclude the role of a gene of interest in relation to a given trait. This chapter will review and discuss the different strategies used for the development of VIGS-based phenomics in model and crop species.

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Authors and Affiliations

  1. Virology and Zoology Section, Science and Advice for Scottish Agriculture (SASA), Roddinglaw Road, Edinburgh, EH12 9FJ, UK

    Christophe Lacomme

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  1. Christophe Lacomme
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Correspondence to Christophe Lacomme .

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Editors and Affiliations

  1. Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA

    Kirankumar S. Mysore

  2. National Institute of Plant Genome Research, New Delhi, Delhi, India

    Muthappa Senthil-Kumar

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Lacomme, C. (2015). Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies. In: Mysore, K., Senthil-Kumar, M. (eds) Plant Gene Silencing. Methods in Molecular Biology, vol 1287. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2453-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2453-0_2

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