Abstract
The G1/S-phase restriction point is an important landmark in the mammalian cell division cycle. The key regulator of the G1/S transition is the retinoblastoma gene product (pRB). It prevents the transcription of genes required for S-phase progression by repressing E2F transcription factors. An increase in Cdk phosphorylation of pRB causes the release of E2F transcription factors and advancement into S phase. Here we describe two simple techniques used to assess pRB phosphorylation and E2F transcription during G1/S progression.
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Thwaites, M.J., Cecchini, M.J., Dick, F.A. (2014). Analyzing RB and E2F During the G1–S Transition. In: Noguchi, E., Gadaleta, M. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 1170. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0888-2_24
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DOI: https://doi.org/10.1007/978-1-4939-0888-2_24
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Publisher Name: Humana Press, New York, NY
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